P-Aminobenzoate synthesis in Escherichia coli: Purification and characterization of PabB as aminodeoxychorismate synthase and enzyme X as aminodeoxychorismate lyase

Qi Zhuang Ye, Jun Liu, Christopher T. Walsh

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The Escherichia coli pabA and pabB genes have been overexpressed separately and in tandem. Using purified PabB, we have confirmed the recent suggestion that PabB needs an additional protein, enzyme X, to convert chorismate and NH3 to p-aminobenzoate (PABA). With chorismate and NH3, pure PabB generates an intermediate presumed to be 4-amino-4-deoxychorismate based upon UV/visible spectroscopy and enzymatic and nonenzymatic transformations. The PabB-catalyzed interconversion of chorismate and isolated aminodeoxychorismate is readily reversible. With pure PabB as a stoichiometric assay reagent, enzyme X was purified ≈800-fold to near homogeneity as an apparent homodimer of 50 kDa from E. coli. Enzyme X shows no activity on chorismate but quantitatively converts the preformed aminodeoxychorismate into p-aminobenzoate and pyruvate, acting thereby as an aminodeoxychorismate lyase.

Original languageEnglish (US)
Pages (from-to)9391-9395
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number23
StatePublished - Jan 1 1990



  • Chorismate
  • Folate biosynthesis

ASJC Scopus subject areas

  • General

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