P-glycoproteins encoded by mdr 1b in murine gravid uterus and multidrug resistant tumor cell lines are differentially glycosylated

Lee M. Greenberger, James M. Croop, Susan Band Horwitz, Robert J. Arceci

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

There are 3 members of the multidrug-resitance gene family expressed in mouse. Only one of these, mdr lb, and its gene product P-glycoprotein are induced to high levels in the mouse endometrium during pregnancy. It is shown here that P-glycoprotein in the gravid uterus is significantly larger (Mr 155000) compared to P-glycoprotein encoded by mdr lb in a murine multidrug-resistant cell line (Mr 140000). However, both species co-migrate after enzymatic removal of N-linked sugars (Mr 125000). These results demonstrate that differential glycosylation of the mdr lb gene product contributes to molecular heterogeneity found in P-glycoprotein from normal and multidrug-resistant cells.

Original languageEnglish (US)
Pages (from-to)419-421
Number of pages3
JournalFEBS Letters
Volume257
Issue number2
DOIs
StatePublished - Nov 6 1989
Externally publishedYes

Keywords

  • Chemotherapy
  • Glycosylation
  • Mouse endometrium
  • Multidrug resistance
  • P-glycoprotein
  • Pregnancy

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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