P16 expression is not associated with human papillomavirus in urinary bladder squamous cell carcinoma

Riley E. Alexander, Yingchuan Hu, Jennifer B. Kum, Rodolfo Montironi, Antonio Lopez-Beltran, Gregory T. MacLennan, Muhammad Idrees, Robert Emerson, Thomas Ulbright, David Grignon, John Eble, Liang Cheng

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Squamous cell carcinoma of the urinary bladder is unusual and of unknown etiology. There is a well-established association between human papillomavirus (HPV) infection and the development of cervical and head/neck squamous cell carcinomas. However, the role of HPV in the pathogenesis of squamous cell carcinoma of the urinary bladder is uncertain. The purposes of this study were to investigate the possible role of HPV in the development of squamous cell carcinoma of the urinary bladder and to determine if p16 expression could serve as a surrogate marker for HPV in this malignancy. In all, 42 cases of squamous cell carcinoma of the urinary bladder and 27 cases of urothelial carcinoma with squamous differentiation were investigated. HPV infection was analyzed by both in situ hybridization at the DNA level and immunohistochemistry at the protein level. p16 protein expression was analyzed by immunohistochemistry. HPV DNA and protein were not detected in 42 cases of squamous cell carcinoma (0%, 0/42) or 27 cases of urothelial carcinoma with squamous differentiation (0%, 0/15). p16 expression was detected in 13 cases (31%, 13/42) of squamous cell carcinoma and 9 cases (33%, 9/27) of urothelial carcinoma with squamous differentiation. There was no correlation between p16 expression and the presence of HPV infection in squamous cell carcinoma of the bladder or urothelial carcinoma with squamous differentiation. Our data suggest that HPV does not play a role in the development of squamous cell carcinoma of the urinary bladder or urothelial carcinoma with squamous differentiation. p16 expression should not be used as a surrogate marker for evidence of HVP infection in either squamous cell carcinoma of the urinary bladder or urothelial carcinoma with squamous differentiation as neither HVP DNA nor protein is detectable in these neoplasms.

Original languageEnglish
Pages (from-to)1526-1533
Number of pages8
JournalModern Pathology
Volume25
Issue number11
DOIs
StatePublished - Nov 2012

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Squamous Cell Carcinoma
Urinary Bladder
Papillomavirus Infections
DNA
Proteins
Biomarkers
Immunohistochemistry
In Situ Hybridization
Neoplasms

Keywords

  • human papillomavirus
  • immunohistochemistry
  • in situ hybridization
  • p16
  • squamous cell carcinoma
  • urinary bladder

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Alexander, R. E., Hu, Y., Kum, J. B., Montironi, R., Lopez-Beltran, A., MacLennan, G. T., ... Cheng, L. (2012). P16 expression is not associated with human papillomavirus in urinary bladder squamous cell carcinoma. Modern Pathology, 25(11), 1526-1533. https://doi.org/10.1038/modpathol.2012.103

P16 expression is not associated with human papillomavirus in urinary bladder squamous cell carcinoma. / Alexander, Riley E.; Hu, Yingchuan; Kum, Jennifer B.; Montironi, Rodolfo; Lopez-Beltran, Antonio; MacLennan, Gregory T.; Idrees, Muhammad; Emerson, Robert; Ulbright, Thomas; Grignon, David; Eble, John; Cheng, Liang.

In: Modern Pathology, Vol. 25, No. 11, 11.2012, p. 1526-1533.

Research output: Contribution to journalArticle

Alexander RE, Hu Y, Kum JB, Montironi R, Lopez-Beltran A, MacLennan GT et al. P16 expression is not associated with human papillomavirus in urinary bladder squamous cell carcinoma. Modern Pathology. 2012 Nov;25(11):1526-1533. https://doi.org/10.1038/modpathol.2012.103
Alexander, Riley E. ; Hu, Yingchuan ; Kum, Jennifer B. ; Montironi, Rodolfo ; Lopez-Beltran, Antonio ; MacLennan, Gregory T. ; Idrees, Muhammad ; Emerson, Robert ; Ulbright, Thomas ; Grignon, David ; Eble, John ; Cheng, Liang. / P16 expression is not associated with human papillomavirus in urinary bladder squamous cell carcinoma. In: Modern Pathology. 2012 ; Vol. 25, No. 11. pp. 1526-1533.
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abstract = "Squamous cell carcinoma of the urinary bladder is unusual and of unknown etiology. There is a well-established association between human papillomavirus (HPV) infection and the development of cervical and head/neck squamous cell carcinomas. However, the role of HPV in the pathogenesis of squamous cell carcinoma of the urinary bladder is uncertain. The purposes of this study were to investigate the possible role of HPV in the development of squamous cell carcinoma of the urinary bladder and to determine if p16 expression could serve as a surrogate marker for HPV in this malignancy. In all, 42 cases of squamous cell carcinoma of the urinary bladder and 27 cases of urothelial carcinoma with squamous differentiation were investigated. HPV infection was analyzed by both in situ hybridization at the DNA level and immunohistochemistry at the protein level. p16 protein expression was analyzed by immunohistochemistry. HPV DNA and protein were not detected in 42 cases of squamous cell carcinoma (0{\%}, 0/42) or 27 cases of urothelial carcinoma with squamous differentiation (0{\%}, 0/15). p16 expression was detected in 13 cases (31{\%}, 13/42) of squamous cell carcinoma and 9 cases (33{\%}, 9/27) of urothelial carcinoma with squamous differentiation. There was no correlation between p16 expression and the presence of HPV infection in squamous cell carcinoma of the bladder or urothelial carcinoma with squamous differentiation. Our data suggest that HPV does not play a role in the development of squamous cell carcinoma of the urinary bladder or urothelial carcinoma with squamous differentiation. p16 expression should not be used as a surrogate marker for evidence of HVP infection in either squamous cell carcinoma of the urinary bladder or urothelial carcinoma with squamous differentiation as neither HVP DNA nor protein is detectable in these neoplasms.",
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