p53 expression in human pancreatic cancer correlates with enhanced biological aggressiveness

M. Yokoyama, Y. Yamanaka, H. Friess, M. Buchler, M. Korc

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Immunohistochemical analysis of the p53 tumor suppressor gene was perfomted in 69 human pancreatic ductal adenocarcinomas, using a highly specific anti-p53 antibody. Nuclear immunoreactivity was found in 40 tumors, yielding an overall frequency of 58%. Immunoblotting confirmed that nuclear immunoreactivity was associated with increased p53 protein levels. p53 mRNA levels were increased in 9 of 9 tested cancers, without evidence for gene amplification. Analysis of the immunostaining data by chi-square and log-ran indicated that the presence of nuclear immunoreacrtivity correlated with a more advanced clinical stage, and a statistically significant decrease in the post-operativesuwival period. In 12 cancers, metastatic tissue samples were also available for p53 analysis. Nuclear p53 immunostaining in the primary atmon was not always associated with p53 immunoreacfivity in the metastatic samples, and metastases occurred in the absence of nuclear p53 immunorenctivity in the piimaly lesion. These findings suggest that increased p53 protein levels in human pancreatic cancer may be due not only to p53 mutations which attenuate the degrandation of the protein but also to an incrense in p53 mRNA levels leading to increased p53 synthesis, and that p53 nuclear immunoreactivity in these cancers implies enhanced tumor aggressiveness but is not essential for the development of metastases.

Original languageEnglish (US)
Pages (from-to)2477-2483
Number of pages7
JournalAnticancer Research
Volume14
Issue number6 B
StatePublished - Dec 1 1994

Keywords

  • Human pancreatic cancer
  • Post-operative survival
  • p53

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Yokoyama, M., Yamanaka, Y., Friess, H., Buchler, M., & Korc, M. (1994). p53 expression in human pancreatic cancer correlates with enhanced biological aggressiveness. Anticancer Research, 14(6 B), 2477-2483.