Pagetic osteoclasts formed in vitro

Absence of paracrystalline inclusions

G. David Roodman, Y. Ohsaki, M. M. Miller, A. Demulder, D. Hosking, F. R. Singer, L. M. McManus

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In Pager's disease of bone, osteoclasts are increased in number and size and contain intracellular paramyxoviral-like inclusions which cross-react with antibody against measles, respiratory syncytial, and canine distemper viral nucleocapsid antigens. Moreover, measles virus nucleocapsid transcripts are present in pagetic osteoclasts and their mononuclear precursors formed in vitro. The present study was undertaken to morphologically assess pagetic osteoclasts formed in culture; special attention has been directed towards the ultrastructural identification of nuclear and cytoplasmic inclusions. Pagetic osteoclasts were produced in long-term cultures of non-adherent bone marrow mononuclear cells derived from involved bone of patients with Paget's disease. These cultured osteoclasts had many of the ultrastructural features of pagetic osteoclasts in vivo. Of interest, no viral-like inclusions were observed in either the multinucleated osteoclasts or mononuclear cell precursors in these cultures. These data suggest that other factors in the bone microenvironment are required for viral nucleocapsid formation in pagetic osteoclasts.

Original languageEnglish (US)
Pages (from-to)315-327
Number of pages13
JournalJournal of Submicroscopic Cytology and Pathology
Volume30
Issue number2
StatePublished - Apr 1998
Externally publishedYes

Fingerprint

Osteoclasts
Nucleocapsid
Distemper
Bone and Bones
Intranuclear Inclusion Bodies
Measles virus
In Vitro Techniques
Viral Antigens
Bone Diseases
Inclusion Bodies
Measles
Bone Marrow Cells
Antibodies

Keywords

  • Bone marrow cultures
  • Osteoclast
  • Paget's disease
  • Ultrastructural

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

Cite this

Roodman, G. D., Ohsaki, Y., Miller, M. M., Demulder, A., Hosking, D., Singer, F. R., & McManus, L. M. (1998). Pagetic osteoclasts formed in vitro: Absence of paracrystalline inclusions. Journal of Submicroscopic Cytology and Pathology, 30(2), 315-327.

Pagetic osteoclasts formed in vitro : Absence of paracrystalline inclusions. / Roodman, G. David; Ohsaki, Y.; Miller, M. M.; Demulder, A.; Hosking, D.; Singer, F. R.; McManus, L. M.

In: Journal of Submicroscopic Cytology and Pathology, Vol. 30, No. 2, 04.1998, p. 315-327.

Research output: Contribution to journalArticle

Roodman, GD, Ohsaki, Y, Miller, MM, Demulder, A, Hosking, D, Singer, FR & McManus, LM 1998, 'Pagetic osteoclasts formed in vitro: Absence of paracrystalline inclusions', Journal of Submicroscopic Cytology and Pathology, vol. 30, no. 2, pp. 315-327.
Roodman GD, Ohsaki Y, Miller MM, Demulder A, Hosking D, Singer FR et al. Pagetic osteoclasts formed in vitro: Absence of paracrystalline inclusions. Journal of Submicroscopic Cytology and Pathology. 1998 Apr;30(2):315-327.
Roodman, G. David ; Ohsaki, Y. ; Miller, M. M. ; Demulder, A. ; Hosking, D. ; Singer, F. R. ; McManus, L. M. / Pagetic osteoclasts formed in vitro : Absence of paracrystalline inclusions. In: Journal of Submicroscopic Cytology and Pathology. 1998 ; Vol. 30, No. 2. pp. 315-327.
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