Pancreatic amylase expression in human pancreatic development

M. M. Davis, M. E. Hodes, R. A. Munsick, T. M. Ulbright, D. J. Goldstein

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Enzymatic and immunologic methods were used to study amylase expression in amniotic fluid and human pancreatic tissue from fetuses of various gestational ages. A starch-coated slide assay was used to quantitate amylase activity in amniotic fluids, and samples with activity were studied by electrophoresis to determine the presence of salivary amylase (Amy1) and pancreatic amylase (Amy2) isozymes. Immunohistochemical studies were performed with both a rabbit anti-human Amy1 antibody and a murine anti-human Amy2 antibody (Amy2/SP2/1). Amy2/SP2/1 was used to discriminate between Amy1 and Amy2. Both Amy1 and Amy2 activities were present in amniotic fluid from 14 weeks' gestation, and immunologic activity was present in pancreatic tissue sections from 15.9 weeks' gestation.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalHybridoma
Volume5
Issue number2
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Immunology
  • Genetics

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