Pancreatic amylase expression in human pancreatic development

M. M. Davis, M. E. Hodes, R. A. Munsick, Thomas Ulbright, D. J. Goldstein

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Enzymatic and immunologic methods were used to study amylase expression in amniotic fluid and human pancreatic tissue from fetuses of various gestational ages. A starch-coated slide assay was used to quantitate amylase activity in amniotic fluids, and samples with activity were studied by electrophoresis to determine the presence of salivary amylase (Amy1) and pancreatic amylase (Amy2) isozymes. Immunohistochemical studies were performed with both a rabbit anti-human Amy1 antibody and a murine anti-human Amy2 antibody (Amy2/SP2/1). Amy2/SP2/1 was used to discriminate between Amy1 and Amy2. Both Amy1 and Amy2 activities were present in amniotic fluid from 14 weeks' gestation, and immunologic activity was present in pancreatic tissue sections from 15.9 weeks' gestation.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalHybridoma
Volume5
Issue number2
StatePublished - 1986
Externally publishedYes

Fingerprint

Human Development
Amylases
Amniotic Fluid
Pregnancy
Antibodies
Starch
Isoenzymes
Gestational Age
Electrophoresis
Fetus
Rabbits

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Davis, M. M., Hodes, M. E., Munsick, R. A., Ulbright, T., & Goldstein, D. J. (1986). Pancreatic amylase expression in human pancreatic development. Hybridoma, 5(2), 137-145.

Pancreatic amylase expression in human pancreatic development. / Davis, M. M.; Hodes, M. E.; Munsick, R. A.; Ulbright, Thomas; Goldstein, D. J.

In: Hybridoma, Vol. 5, No. 2, 1986, p. 137-145.

Research output: Contribution to journalArticle

Davis, MM, Hodes, ME, Munsick, RA, Ulbright, T & Goldstein, DJ 1986, 'Pancreatic amylase expression in human pancreatic development', Hybridoma, vol. 5, no. 2, pp. 137-145.
Davis MM, Hodes ME, Munsick RA, Ulbright T, Goldstein DJ. Pancreatic amylase expression in human pancreatic development. Hybridoma. 1986;5(2):137-145.
Davis, M. M. ; Hodes, M. E. ; Munsick, R. A. ; Ulbright, Thomas ; Goldstein, D. J. / Pancreatic amylase expression in human pancreatic development. In: Hybridoma. 1986 ; Vol. 5, No. 2. pp. 137-145.
@article{5e92322a9a7c4b69bcfd05b7a83bea14,
title = "Pancreatic amylase expression in human pancreatic development",
abstract = "Enzymatic and immunologic methods were used to study amylase expression in amniotic fluid and human pancreatic tissue from fetuses of various gestational ages. A starch-coated slide assay was used to quantitate amylase activity in amniotic fluids, and samples with activity were studied by electrophoresis to determine the presence of salivary amylase (Amy1) and pancreatic amylase (Amy2) isozymes. Immunohistochemical studies were performed with both a rabbit anti-human Amy1 antibody and a murine anti-human Amy2 antibody (Amy2/SP2/1). Amy2/SP2/1 was used to discriminate between Amy1 and Amy2. Both Amy1 and Amy2 activities were present in amniotic fluid from 14 weeks' gestation, and immunologic activity was present in pancreatic tissue sections from 15.9 weeks' gestation.",
author = "Davis, {M. M.} and Hodes, {M. E.} and Munsick, {R. A.} and Thomas Ulbright and Goldstein, {D. J.}",
year = "1986",
language = "English (US)",
volume = "5",
pages = "137--145",
journal = "Monoclonal Antibodies in Immunodiagnosis and Immunotherapy",
issn = "2167-9436",
publisher = "Mary Ann Liebert Inc.",
number = "2",

}

TY - JOUR

T1 - Pancreatic amylase expression in human pancreatic development

AU - Davis, M. M.

AU - Hodes, M. E.

AU - Munsick, R. A.

AU - Ulbright, Thomas

AU - Goldstein, D. J.

PY - 1986

Y1 - 1986

N2 - Enzymatic and immunologic methods were used to study amylase expression in amniotic fluid and human pancreatic tissue from fetuses of various gestational ages. A starch-coated slide assay was used to quantitate amylase activity in amniotic fluids, and samples with activity were studied by electrophoresis to determine the presence of salivary amylase (Amy1) and pancreatic amylase (Amy2) isozymes. Immunohistochemical studies were performed with both a rabbit anti-human Amy1 antibody and a murine anti-human Amy2 antibody (Amy2/SP2/1). Amy2/SP2/1 was used to discriminate between Amy1 and Amy2. Both Amy1 and Amy2 activities were present in amniotic fluid from 14 weeks' gestation, and immunologic activity was present in pancreatic tissue sections from 15.9 weeks' gestation.

AB - Enzymatic and immunologic methods were used to study amylase expression in amniotic fluid and human pancreatic tissue from fetuses of various gestational ages. A starch-coated slide assay was used to quantitate amylase activity in amniotic fluids, and samples with activity were studied by electrophoresis to determine the presence of salivary amylase (Amy1) and pancreatic amylase (Amy2) isozymes. Immunohistochemical studies were performed with both a rabbit anti-human Amy1 antibody and a murine anti-human Amy2 antibody (Amy2/SP2/1). Amy2/SP2/1 was used to discriminate between Amy1 and Amy2. Both Amy1 and Amy2 activities were present in amniotic fluid from 14 weeks' gestation, and immunologic activity was present in pancreatic tissue sections from 15.9 weeks' gestation.

UR - http://www.scopus.com/inward/record.url?scp=0022497301&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022497301&partnerID=8YFLogxK

M3 - Article

C2 - 2424823

AN - SCOPUS:0022497301

VL - 5

SP - 137

EP - 145

JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

SN - 2167-9436

IS - 2

ER -