Papillomavirus-associated inductions of cellular proteins in mouse C127 cells: Correlation with the presence of open reading frame E2

Richard M. Levenson, Ute G. Brinckmann, M. Kerry O'Banion, Elliot Androphy, John T. Schiller, Fareed Tabatabai, Lubomir P.Turek, Kathryn Neary, Michael T. Chin, Thomas R. Broker, Louise T. Chow, Donald A. Oung

Research output: Contribution to journalArticle

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Abstract

Bovine papillomavirus type 1 (BPV-1) readily transforms mouse C127 cells, conferring the ability to grow in soft agar and to form tumors in athymic (nu/nu) mice. Electrophoresis of total cellular proteins from these BPV-transformed lines on ultra-high resolution, giant two-dimensional gels displays the presence of novel, papillomavirus-related protein phenotypes. Analysis of the established BPV-1-transformed C127 cell lines, ID13 and ID14, reveals a set of six proteins which are either absent or synthesized at extremely low levels in the parental cell line. One of these proteins is also present in v-ras-transformed C127 cells, but none of the others are found in cells transformed by a variety of viral oncogenes, including the polyomavirus middle T, v-mos, or v-fes. The genome of BPV-1 contains two separate open reading frames (ORFs), E5 and E6, which can act independently to transform C127 cells. In addition, trans-activator and repressor proteins encoded respectively by the full-length and carboxy-terminal E2 ORF regulate the level of expression of other BPV-1 genes. We examined 34 cell lines transformed by intact and subgenomic recombinant DNAs of BPV-1. Cells harboring BPV-1 DNAs engineered to eliminate the expression of ORFs E4, E5, E6, or E7 display five of the PV-associated proteins, but these proteins are not seen in lines lacking the full E2 ORF. Moreover, G418-selected nontransformed cells expressing E2 cDNA from an SV40 promoter exhibit these proteins at high levels. Surprisingly, these proteins are also present in cells containing BPV-1 DNAs with amino-terminal E2 deletions, suggesting that these PV-associated proteins represent novel cellular responses to a factor encoded within the E2-C gene region.

Original languageEnglish (US)
Pages (from-to)170-179
Number of pages10
JournalVirology
Volume172
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Bovine papillomavirus 1
Open Reading Frames
Proteins
Transformed Cell Line
Repressor Proteins
Polyomavirus
Trans-Activators
Recombinant DNA
DNA
Oncogenes
Genes
Agar
Electrophoresis
Complementary DNA
Gels
Genome
Phenotype
Cell Line

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Levenson, R. M., Brinckmann, U. G., Kerry O'Banion, M., Androphy, E., Schiller, J. T., Tabatabai, F., ... Oung, D. A. (1989). Papillomavirus-associated inductions of cellular proteins in mouse C127 cells: Correlation with the presence of open reading frame E2. Virology, 172(1), 170-179. https://doi.org/10.1016/0042-6822(89)90118-9

Papillomavirus-associated inductions of cellular proteins in mouse C127 cells : Correlation with the presence of open reading frame E2. / Levenson, Richard M.; Brinckmann, Ute G.; Kerry O'Banion, M.; Androphy, Elliot; Schiller, John T.; Tabatabai, Fareed; P.Turek, Lubomir; Neary, Kathryn; Chin, Michael T.; Broker, Thomas R.; Chow, Louise T.; Oung, Donald A.

In: Virology, Vol. 172, No. 1, 1989, p. 170-179.

Research output: Contribution to journalArticle

Levenson, RM, Brinckmann, UG, Kerry O'Banion, M, Androphy, E, Schiller, JT, Tabatabai, F, P.Turek, L, Neary, K, Chin, MT, Broker, TR, Chow, LT & Oung, DA 1989, 'Papillomavirus-associated inductions of cellular proteins in mouse C127 cells: Correlation with the presence of open reading frame E2', Virology, vol. 172, no. 1, pp. 170-179. https://doi.org/10.1016/0042-6822(89)90118-9
Levenson, Richard M. ; Brinckmann, Ute G. ; Kerry O'Banion, M. ; Androphy, Elliot ; Schiller, John T. ; Tabatabai, Fareed ; P.Turek, Lubomir ; Neary, Kathryn ; Chin, Michael T. ; Broker, Thomas R. ; Chow, Louise T. ; Oung, Donald A. / Papillomavirus-associated inductions of cellular proteins in mouse C127 cells : Correlation with the presence of open reading frame E2. In: Virology. 1989 ; Vol. 172, No. 1. pp. 170-179.
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abstract = "Bovine papillomavirus type 1 (BPV-1) readily transforms mouse C127 cells, conferring the ability to grow in soft agar and to form tumors in athymic (nu/nu) mice. Electrophoresis of total cellular proteins from these BPV-transformed lines on ultra-high resolution, giant two-dimensional gels displays the presence of novel, papillomavirus-related protein phenotypes. Analysis of the established BPV-1-transformed C127 cell lines, ID13 and ID14, reveals a set of six proteins which are either absent or synthesized at extremely low levels in the parental cell line. One of these proteins is also present in v-ras-transformed C127 cells, but none of the others are found in cells transformed by a variety of viral oncogenes, including the polyomavirus middle T, v-mos, or v-fes. The genome of BPV-1 contains two separate open reading frames (ORFs), E5 and E6, which can act independently to transform C127 cells. In addition, trans-activator and repressor proteins encoded respectively by the full-length and carboxy-terminal E2 ORF regulate the level of expression of other BPV-1 genes. We examined 34 cell lines transformed by intact and subgenomic recombinant DNAs of BPV-1. Cells harboring BPV-1 DNAs engineered to eliminate the expression of ORFs E4, E5, E6, or E7 display five of the PV-associated proteins, but these proteins are not seen in lines lacking the full E2 ORF. Moreover, G418-selected nontransformed cells expressing E2 cDNA from an SV40 promoter exhibit these proteins at high levels. Surprisingly, these proteins are also present in cells containing BPV-1 DNAs with amino-terminal E2 deletions, suggesting that these PV-associated proteins represent novel cellular responses to a factor encoded within the E2-C gene region.",
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AU - Androphy, Elliot

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