Papillomavirus E2 protein is regulated by specific fibroblast growth factor receptors

Marsha DeSmet, Sriramana Kanginakudru, Leny Jose, Fang Xie, Timra Gilson, Elliot Androphy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The papillomavirus (PV) E2 protein activates transcription and replication by recruiting cellular proteins and the E1 DNA helicase to their binding sites in the viral genome. We recently demonstrated that phosphorylation of tyrosine 102 in the bovine papillomavirus (BPV-1) E2 protein restricts these activities and that fibroblast growth factor receptor-3 (FGFR3) tyrosine kinase binds PV E2. Expression of FGFR3 decreased viral replication with both wild-type and the phenylalanine substitution at position 102, inferring that another kinase targets Y102. Here we tested FGFR- 1, −2 and −4 for association with PV E2 proteins. FGFR2 but not FGFR1 or FGFR4 co-immunoprecipitated with BPV-1 E2. We found that FGFR2 suppressed replication but did not depend on phosphorylation of BPV-1 Y102. HPV-16 and −31 E2 interacted with FGFR1, −2, and −4. These results imply that the expression and activity of FGF receptors in epithelial cells can regulate the function of E2 in viral replication.

Original languageEnglish (US)
Pages (from-to)62-68
Number of pages7
JournalVirology
Volume521
DOIs
StatePublished - Aug 1 2018

Fingerprint

Fibroblast Growth Factor Receptors
Receptor, Fibroblast Growth Factor, Type 3
Human papillomavirus 31
Phosphorylation
DNA Helicases
Proteins
Human papillomavirus 16
Viral Genome
Phenylalanine
Protein-Tyrosine Kinases
Tyrosine
Phosphotransferases
Epithelial Cells
Binding Sites

Keywords

  • Fibroblast growth factor receptor
  • Papillomavirus E2 phosphorylation
  • Viral replication

ASJC Scopus subject areas

  • Virology

Cite this

Papillomavirus E2 protein is regulated by specific fibroblast growth factor receptors. / DeSmet, Marsha; Kanginakudru, Sriramana; Jose, Leny; Xie, Fang; Gilson, Timra; Androphy, Elliot.

In: Virology, Vol. 521, 01.08.2018, p. 62-68.

Research output: Contribution to journalArticle

DeSmet, Marsha ; Kanginakudru, Sriramana ; Jose, Leny ; Xie, Fang ; Gilson, Timra ; Androphy, Elliot. / Papillomavirus E2 protein is regulated by specific fibroblast growth factor receptors. In: Virology. 2018 ; Vol. 521. pp. 62-68.
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