Paracrine IL-2 is required for optimal type 2 effector cytokine production

Matthew R. Olson, Benjamin J. Ulrich, Sarah A. Hummel, Ibrahim Khan, Brice Meuris, Yesesri Cherukuri, Alexander Dent, Sarath Chandra Janga, Mark Kaplan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

IL-2 is a pleiotropic cytokine that promotes the differentiation of Th cell subsets, including Th1, Th2, and Th9 cells, but it impairs the development of Th17 and T follicular helper cells. Although IL-2 is produced by all polarized Th subsets to some level, how it impacts cytokine production when effector T cells are restimulated is unknown. We show in this article that Golgi transport inhibitors (GTIs) blocked IL-9 production. Mechanistically, GTIs blocked secretion of IL-2 that normally feeds back in a paracrine manner to promote STAT5 activation and IL-9 production. IL-2 feedback had no effect on Th1- or Th17-signature cytokine production, but it promoted Th2- and Th9-associated cytokine expression. These data suggest that the use of GTIs results in an underestimation of the presence of type 2 cytokine-secreting cells and highlight IL-2 as a critical component in optimal cytokine production by Th2 and Th9 cells in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)4352-4359
Number of pages8
JournalJournal of Immunology
Volume198
Issue number11
DOIs
StatePublished - Jun 1 2017

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Interleukin-2
Cytokines
Interleukin-9
Th2 Cells
Helper-Inducer T-Lymphocytes
Cell Differentiation
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Olson, M. R., Ulrich, B. J., Hummel, S. A., Khan, I., Meuris, B., Cherukuri, Y., ... Kaplan, M. (2017). Paracrine IL-2 is required for optimal type 2 effector cytokine production. Journal of Immunology, 198(11), 4352-4359. https://doi.org/10.4049/jimmunol.1601792

Paracrine IL-2 is required for optimal type 2 effector cytokine production. / Olson, Matthew R.; Ulrich, Benjamin J.; Hummel, Sarah A.; Khan, Ibrahim; Meuris, Brice; Cherukuri, Yesesri; Dent, Alexander; Janga, Sarath Chandra; Kaplan, Mark.

In: Journal of Immunology, Vol. 198, No. 11, 01.06.2017, p. 4352-4359.

Research output: Contribution to journalArticle

Olson, MR, Ulrich, BJ, Hummel, SA, Khan, I, Meuris, B, Cherukuri, Y, Dent, A, Janga, SC & Kaplan, M 2017, 'Paracrine IL-2 is required for optimal type 2 effector cytokine production', Journal of Immunology, vol. 198, no. 11, pp. 4352-4359. https://doi.org/10.4049/jimmunol.1601792
Olson MR, Ulrich BJ, Hummel SA, Khan I, Meuris B, Cherukuri Y et al. Paracrine IL-2 is required for optimal type 2 effector cytokine production. Journal of Immunology. 2017 Jun 1;198(11):4352-4359. https://doi.org/10.4049/jimmunol.1601792
Olson, Matthew R. ; Ulrich, Benjamin J. ; Hummel, Sarah A. ; Khan, Ibrahim ; Meuris, Brice ; Cherukuri, Yesesri ; Dent, Alexander ; Janga, Sarath Chandra ; Kaplan, Mark. / Paracrine IL-2 is required for optimal type 2 effector cytokine production. In: Journal of Immunology. 2017 ; Vol. 198, No. 11. pp. 4352-4359.
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