Paracrine stimulation of preadipocyte-enriched cell cultures by mature adipocytes

Robert Considine, Mark R. Nyce, Luz Marina Morales, Susan A. Magosin, Madhur K. Sinha, Thomas L. Bauer, Ernest L. Rosato, James Colberg, Jose F. Caro

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We developed a double-chamber system in which to examine the effects of mature adipocytes on the growth and differentiation of preadipocytes and other cells in the adipose tissue. In the present study we found that mature adipocytes from both lean and obese subjects release a factor that stimulates the growth of preadipocyte-enriched and dedifferentiated adipocyte-enriched cell cultures. This growth stimulation was dependent on both time of exposure to mature cells and the number of mature cells in the coculture. Proliferation of the preadipocyte-enriched (n = 4) and dedifferentiated adipocyte-enriched cultures (n = 5) in the presence of mature adipocytes from obese subjects [body mass index (BMI) >35] was 4.1- and 2.9-fold more (P <0.05) than that in the presence of adipocytes from lean subjects (BMI ≤25). There was no difference in the growth of cultures enriched in preadipocytes or dedifferentiated adipocytes from lean or obese subjects in the absence of mature adipocytes. These observations demonstrate that mature adipocytes from obese patients stimulate the growth of preadipocyte-enriched cultures to a greater extent than those from lean individuals.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume270
Issue number5 33-5
StatePublished - 1996
Externally publishedYes

Fingerprint

Cell culture
Adipocytes
Cell Culture Techniques
Growth
Intercellular Signaling Peptides and Proteins
Body Mass Index
Tissue
Coculture Techniques
Adipose Tissue
Cell Count

Keywords

  • adipose tissue
  • dedifferentiated adipocytes

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Considine, R., Nyce, M. R., Morales, L. M., Magosin, S. A., Sinha, M. K., Bauer, T. L., ... Caro, J. F. (1996). Paracrine stimulation of preadipocyte-enriched cell cultures by mature adipocytes. American Journal of Physiology - Endocrinology and Metabolism, 270(5 33-5).

Paracrine stimulation of preadipocyte-enriched cell cultures by mature adipocytes. / Considine, Robert; Nyce, Mark R.; Morales, Luz Marina; Magosin, Susan A.; Sinha, Madhur K.; Bauer, Thomas L.; Rosato, Ernest L.; Colberg, James; Caro, Jose F.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 270, No. 5 33-5, 1996.

Research output: Contribution to journalArticle

Considine, R, Nyce, MR, Morales, LM, Magosin, SA, Sinha, MK, Bauer, TL, Rosato, EL, Colberg, J & Caro, JF 1996, 'Paracrine stimulation of preadipocyte-enriched cell cultures by mature adipocytes', American Journal of Physiology - Endocrinology and Metabolism, vol. 270, no. 5 33-5.
Considine, Robert ; Nyce, Mark R. ; Morales, Luz Marina ; Magosin, Susan A. ; Sinha, Madhur K. ; Bauer, Thomas L. ; Rosato, Ernest L. ; Colberg, James ; Caro, Jose F. / Paracrine stimulation of preadipocyte-enriched cell cultures by mature adipocytes. In: American Journal of Physiology - Endocrinology and Metabolism. 1996 ; Vol. 270, No. 5 33-5.
@article{1850c263a30b4bed8fc55e97a7c63d0e,
title = "Paracrine stimulation of preadipocyte-enriched cell cultures by mature adipocytes",
abstract = "We developed a double-chamber system in which to examine the effects of mature adipocytes on the growth and differentiation of preadipocytes and other cells in the adipose tissue. In the present study we found that mature adipocytes from both lean and obese subjects release a factor that stimulates the growth of preadipocyte-enriched and dedifferentiated adipocyte-enriched cell cultures. This growth stimulation was dependent on both time of exposure to mature cells and the number of mature cells in the coculture. Proliferation of the preadipocyte-enriched (n = 4) and dedifferentiated adipocyte-enriched cultures (n = 5) in the presence of mature adipocytes from obese subjects [body mass index (BMI) >35] was 4.1- and 2.9-fold more (P <0.05) than that in the presence of adipocytes from lean subjects (BMI ≤25). There was no difference in the growth of cultures enriched in preadipocytes or dedifferentiated adipocytes from lean or obese subjects in the absence of mature adipocytes. These observations demonstrate that mature adipocytes from obese patients stimulate the growth of preadipocyte-enriched cultures to a greater extent than those from lean individuals.",
keywords = "adipose tissue, dedifferentiated adipocytes",
author = "Robert Considine and Nyce, {Mark R.} and Morales, {Luz Marina} and Magosin, {Susan A.} and Sinha, {Madhur K.} and Bauer, {Thomas L.} and Rosato, {Ernest L.} and James Colberg and Caro, {Jose F.}",
year = "1996",
language = "English (US)",
volume = "270",
journal = "American Journal of Physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "5 33-5",

}

TY - JOUR

T1 - Paracrine stimulation of preadipocyte-enriched cell cultures by mature adipocytes

AU - Considine, Robert

AU - Nyce, Mark R.

AU - Morales, Luz Marina

AU - Magosin, Susan A.

AU - Sinha, Madhur K.

AU - Bauer, Thomas L.

AU - Rosato, Ernest L.

AU - Colberg, James

AU - Caro, Jose F.

PY - 1996

Y1 - 1996

N2 - We developed a double-chamber system in which to examine the effects of mature adipocytes on the growth and differentiation of preadipocytes and other cells in the adipose tissue. In the present study we found that mature adipocytes from both lean and obese subjects release a factor that stimulates the growth of preadipocyte-enriched and dedifferentiated adipocyte-enriched cell cultures. This growth stimulation was dependent on both time of exposure to mature cells and the number of mature cells in the coculture. Proliferation of the preadipocyte-enriched (n = 4) and dedifferentiated adipocyte-enriched cultures (n = 5) in the presence of mature adipocytes from obese subjects [body mass index (BMI) >35] was 4.1- and 2.9-fold more (P <0.05) than that in the presence of adipocytes from lean subjects (BMI ≤25). There was no difference in the growth of cultures enriched in preadipocytes or dedifferentiated adipocytes from lean or obese subjects in the absence of mature adipocytes. These observations demonstrate that mature adipocytes from obese patients stimulate the growth of preadipocyte-enriched cultures to a greater extent than those from lean individuals.

AB - We developed a double-chamber system in which to examine the effects of mature adipocytes on the growth and differentiation of preadipocytes and other cells in the adipose tissue. In the present study we found that mature adipocytes from both lean and obese subjects release a factor that stimulates the growth of preadipocyte-enriched and dedifferentiated adipocyte-enriched cell cultures. This growth stimulation was dependent on both time of exposure to mature cells and the number of mature cells in the coculture. Proliferation of the preadipocyte-enriched (n = 4) and dedifferentiated adipocyte-enriched cultures (n = 5) in the presence of mature adipocytes from obese subjects [body mass index (BMI) >35] was 4.1- and 2.9-fold more (P <0.05) than that in the presence of adipocytes from lean subjects (BMI ≤25). There was no difference in the growth of cultures enriched in preadipocytes or dedifferentiated adipocytes from lean or obese subjects in the absence of mature adipocytes. These observations demonstrate that mature adipocytes from obese patients stimulate the growth of preadipocyte-enriched cultures to a greater extent than those from lean individuals.

KW - adipose tissue

KW - dedifferentiated adipocytes

UR - http://www.scopus.com/inward/record.url?scp=0029942345&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029942345&partnerID=8YFLogxK

M3 - Article

C2 - 8967480

AN - SCOPUS:0029942345

VL - 270

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1857

IS - 5 33-5

ER -