Paradoxical effect of IKKβ inhibition on the expression of E3 ubiquitin ligases and unloading-induced skeletal muscle atrophy

Svetlana P. Belova, Boris S. Shenkman, Tatiana Y. Kostrominova, Tatiana L. Nemirovskaya

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We tested whether NF-κB pathway is indispensable for the increase in expression of E3-ligases and unloading-induced muscle atrophy using IKKβ inhibitor IMD-0354. Three groups of rats were used: nontreated control (C), 3 days of unloading/hindlimb suspension with (HS+IMD) or without (HS) IMD-0354. Levels of IκBα were higher in HS+IMD (1.16-fold) and lower in HS (0.82-fold) when compared with C group. IMD-0354 treatment during unloading: had no effect on loss of muscle mass; increased mRNA levels of MuRF1 and MAFbx; increased levels of pFoxO3; and had no effect on levels of Bcl-3, p105, and p50 proteins. Our study for the first time showed that inhibiting IKKβ in vivo during 3-day unloading failed to diminish expression of ubiquitin ligases and prevent muscle atrophy.

Original languageEnglish (US)
Article numbere13291
JournalPhysiological reports
Volume5
Issue number16
DOIs
StatePublished - Aug 2017

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Keywords

  • FoxO3
  • MAFbx
  • MuRF1
  • nuclear factor-κB
  • skeletal muscle
  • unloading

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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