Parathyroid hormone

Sequence, synthesis, immunoassay studies

J. T. Potts, T. M. Murray, Munro Peacock, H. D. Niall, G. W. Tregear, H. T. Keutmann, D. Powell, L. J. Deftos

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The determination of the primary structure of bovine parathyroid hormone (PTH) has enabled us to carry out the synthesis of the first N-terminal 34 amino acids of the molecule. This synthetic fragment has both phosphaturic and hypercalcemic activity, confirming that the biologic actions of PTH are expressed by a short sequence of the "native" 84 amino acid sequence. Serum immunoreactive PTH of patients with surgically proved parathyroid adenomas decreases with induced hypercalcemia and increases in response to hypocalcemia caused by EDTA infusions. These findings challenge the concept of parathyroid adenoma autonomy. Further studies of selective venous catheterization and immunoassay of serum samples obtained from various sites have not been as successful in the localization of parathyroid lesions as originally reported, but newer technics designed to obtain blood from the thyroid venous effluent show promise. Evidence is presented which confirms the concept of immunochemical heterogeneity of PTH in human serum.

Original languageEnglish (US)
Pages (from-to)639-649
Number of pages11
JournalThe American Journal of Medicine
Volume50
Issue number5
DOIs
StatePublished - Jan 1 1971
Externally publishedYes

Fingerprint

Parathyroid Hormone
Immunoassay
Parathyroid Neoplasms
Serum
Hypocalcemia
Hypercalcemia
Edetic Acid
Catheterization
Amino Acid Sequence
Thyroid Gland
Amino Acids

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Potts, J. T., Murray, T. M., Peacock, M., Niall, H. D., Tregear, G. W., Keutmann, H. T., ... Deftos, L. J. (1971). Parathyroid hormone: Sequence, synthesis, immunoassay studies. The American Journal of Medicine, 50(5), 639-649. https://doi.org/10.1016/0002-9343(71)90119-7

Parathyroid hormone : Sequence, synthesis, immunoassay studies. / Potts, J. T.; Murray, T. M.; Peacock, Munro; Niall, H. D.; Tregear, G. W.; Keutmann, H. T.; Powell, D.; Deftos, L. J.

In: The American Journal of Medicine, Vol. 50, No. 5, 01.01.1971, p. 639-649.

Research output: Contribution to journalArticle

Potts, JT, Murray, TM, Peacock, M, Niall, HD, Tregear, GW, Keutmann, HT, Powell, D & Deftos, LJ 1971, 'Parathyroid hormone: Sequence, synthesis, immunoassay studies', The American Journal of Medicine, vol. 50, no. 5, pp. 639-649. https://doi.org/10.1016/0002-9343(71)90119-7
Potts, J. T. ; Murray, T. M. ; Peacock, Munro ; Niall, H. D. ; Tregear, G. W. ; Keutmann, H. T. ; Powell, D. ; Deftos, L. J. / Parathyroid hormone : Sequence, synthesis, immunoassay studies. In: The American Journal of Medicine. 1971 ; Vol. 50, No. 5. pp. 639-649.
@article{5d42776bbe3d4196b7fa7cc8d4701acf,
title = "Parathyroid hormone: Sequence, synthesis, immunoassay studies",
abstract = "The determination of the primary structure of bovine parathyroid hormone (PTH) has enabled us to carry out the synthesis of the first N-terminal 34 amino acids of the molecule. This synthetic fragment has both phosphaturic and hypercalcemic activity, confirming that the biologic actions of PTH are expressed by a short sequence of the {"}native{"} 84 amino acid sequence. Serum immunoreactive PTH of patients with surgically proved parathyroid adenomas decreases with induced hypercalcemia and increases in response to hypocalcemia caused by EDTA infusions. These findings challenge the concept of parathyroid adenoma autonomy. Further studies of selective venous catheterization and immunoassay of serum samples obtained from various sites have not been as successful in the localization of parathyroid lesions as originally reported, but newer technics designed to obtain blood from the thyroid venous effluent show promise. Evidence is presented which confirms the concept of immunochemical heterogeneity of PTH in human serum.",
author = "Potts, {J. T.} and Murray, {T. M.} and Munro Peacock and Niall, {H. D.} and Tregear, {G. W.} and Keutmann, {H. T.} and D. Powell and Deftos, {L. J.}",
year = "1971",
month = "1",
day = "1",
doi = "10.1016/0002-9343(71)90119-7",
language = "English (US)",
volume = "50",
pages = "639--649",
journal = "American Journal of Medicine",
issn = "0002-9343",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Parathyroid hormone

T2 - Sequence, synthesis, immunoassay studies

AU - Potts, J. T.

AU - Murray, T. M.

AU - Peacock, Munro

AU - Niall, H. D.

AU - Tregear, G. W.

AU - Keutmann, H. T.

AU - Powell, D.

AU - Deftos, L. J.

PY - 1971/1/1

Y1 - 1971/1/1

N2 - The determination of the primary structure of bovine parathyroid hormone (PTH) has enabled us to carry out the synthesis of the first N-terminal 34 amino acids of the molecule. This synthetic fragment has both phosphaturic and hypercalcemic activity, confirming that the biologic actions of PTH are expressed by a short sequence of the "native" 84 amino acid sequence. Serum immunoreactive PTH of patients with surgically proved parathyroid adenomas decreases with induced hypercalcemia and increases in response to hypocalcemia caused by EDTA infusions. These findings challenge the concept of parathyroid adenoma autonomy. Further studies of selective venous catheterization and immunoassay of serum samples obtained from various sites have not been as successful in the localization of parathyroid lesions as originally reported, but newer technics designed to obtain blood from the thyroid venous effluent show promise. Evidence is presented which confirms the concept of immunochemical heterogeneity of PTH in human serum.

AB - The determination of the primary structure of bovine parathyroid hormone (PTH) has enabled us to carry out the synthesis of the first N-terminal 34 amino acids of the molecule. This synthetic fragment has both phosphaturic and hypercalcemic activity, confirming that the biologic actions of PTH are expressed by a short sequence of the "native" 84 amino acid sequence. Serum immunoreactive PTH of patients with surgically proved parathyroid adenomas decreases with induced hypercalcemia and increases in response to hypocalcemia caused by EDTA infusions. These findings challenge the concept of parathyroid adenoma autonomy. Further studies of selective venous catheterization and immunoassay of serum samples obtained from various sites have not been as successful in the localization of parathyroid lesions as originally reported, but newer technics designed to obtain blood from the thyroid venous effluent show promise. Evidence is presented which confirms the concept of immunochemical heterogeneity of PTH in human serum.

UR - http://www.scopus.com/inward/record.url?scp=0015056209&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015056209&partnerID=8YFLogxK

U2 - 10.1016/0002-9343(71)90119-7

DO - 10.1016/0002-9343(71)90119-7

M3 - Article

VL - 50

SP - 639

EP - 649

JO - American Journal of Medicine

JF - American Journal of Medicine

SN - 0002-9343

IS - 5

ER -