Partial protection against genital reinfection by immunization of guinea-pigs with isolated outer-membrane proteins of the chlamydial agent of guinea-pig inclusion conjunctivitis

Byron Batteiger, R. G. Rank, P. M. Bavoil, L. S F Soderberg

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Abstract

Because partial protection against reinfection is induced by experimental infection in the guinea-pig model of genital chlamydial infection, we sought to induce immunity by immunization. Female guinea-pigs were immunized subcutaneously with the major outer-membrane protein (MOMP) and the 61 kDa cysteine-rich outer-membrane protein (61 kDa) of the agent of guinea-pig inclusion conjunctivitis (GPIC) eluted from SDS-polyacrylamide gels (SDS-MOMP, SDS-61 kDa). Post-immunization sera and secretions contained antibodies to the SDS-purified proteins at high titre as measured by immunoblotting, whereas enzyme immunoassays (EIA) using whole elementary bodies as antigen showed significantly lower titres (P < 0.001). Likewise, blastogenic responses of peripheral mononuclear cells to GPIC elementary bodies were weak. Animals immunized with SDS-MOMP and SDS-61 kDa were fully susceptible to intravaginal challenge, as were control animals immunized with buffer without protein. Another group of animals were immunized with material prepared by extraction of chlamydial outer-membrane complexes with octyl β-D-glucopyranoside (OGP) and dithiothreitol, which consisted largely of MOMP (OGP-MOMP). In contrast to the SDS-MOMP group, sera and secretions in the OGP-MOMP group showed high titres in EIA, and high titre antibodies to MOMP by immunoblot; however, most animals also had antibodies to 61 kDa, 72 kDa and ca. 84 kDa outer-membrane proteins. OGP-MOMP animals were partially protected against genital challenge as evidenced by low inclusion scores compared to control animals, although duration of infection measured by culture isolation was similar to controls. Immunoblot analysis of sera from immunized animals and from a group of immune animals post-infection was performed using recombinant fusion peptides containing the four variable domains of MOMP. No consistent differences in reaction patterns were observed when sera from protected and non-protected animals were compared. Thus, a highly refined outer-membrane preparation is capable of producing partial immunity to genital infection. Further study is required to determine whether the protection is due to MOMP itself or to other outer-membrane proteins found in small amounts in the OGP-MOMP immunogen. The results suggest the possibility that discontinuous MOMP epitopes could play a role in inducing a protective immune response in the guinea-pig model, a concept that requires further evaluation.

Original languageEnglish
Pages (from-to)2965-2972
Number of pages8
JournalJournal of General Microbiology
Volume139
Issue number12
StatePublished - 1993

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Inclusion Conjunctivitis
Immunization
Guinea Pigs
Membrane Proteins
Infection
Serum
Immunoenzyme Techniques
Antibodies
Immunity

ASJC Scopus subject areas

  • Microbiology

Cite this

@article{aeb8ee3145f946c1b39c9365ebe6e1b8,
title = "Partial protection against genital reinfection by immunization of guinea-pigs with isolated outer-membrane proteins of the chlamydial agent of guinea-pig inclusion conjunctivitis",
abstract = "Because partial protection against reinfection is induced by experimental infection in the guinea-pig model of genital chlamydial infection, we sought to induce immunity by immunization. Female guinea-pigs were immunized subcutaneously with the major outer-membrane protein (MOMP) and the 61 kDa cysteine-rich outer-membrane protein (61 kDa) of the agent of guinea-pig inclusion conjunctivitis (GPIC) eluted from SDS-polyacrylamide gels (SDS-MOMP, SDS-61 kDa). Post-immunization sera and secretions contained antibodies to the SDS-purified proteins at high titre as measured by immunoblotting, whereas enzyme immunoassays (EIA) using whole elementary bodies as antigen showed significantly lower titres (P < 0.001). Likewise, blastogenic responses of peripheral mononuclear cells to GPIC elementary bodies were weak. Animals immunized with SDS-MOMP and SDS-61 kDa were fully susceptible to intravaginal challenge, as were control animals immunized with buffer without protein. Another group of animals were immunized with material prepared by extraction of chlamydial outer-membrane complexes with octyl β-D-glucopyranoside (OGP) and dithiothreitol, which consisted largely of MOMP (OGP-MOMP). In contrast to the SDS-MOMP group, sera and secretions in the OGP-MOMP group showed high titres in EIA, and high titre antibodies to MOMP by immunoblot; however, most animals also had antibodies to 61 kDa, 72 kDa and ca. 84 kDa outer-membrane proteins. OGP-MOMP animals were partially protected against genital challenge as evidenced by low inclusion scores compared to control animals, although duration of infection measured by culture isolation was similar to controls. Immunoblot analysis of sera from immunized animals and from a group of immune animals post-infection was performed using recombinant fusion peptides containing the four variable domains of MOMP. No consistent differences in reaction patterns were observed when sera from protected and non-protected animals were compared. Thus, a highly refined outer-membrane preparation is capable of producing partial immunity to genital infection. Further study is required to determine whether the protection is due to MOMP itself or to other outer-membrane proteins found in small amounts in the OGP-MOMP immunogen. The results suggest the possibility that discontinuous MOMP epitopes could play a role in inducing a protective immune response in the guinea-pig model, a concept that requires further evaluation.",
author = "Byron Batteiger and Rank, {R. G.} and Bavoil, {P. M.} and Soderberg, {L. S F}",
year = "1993",
language = "English",
volume = "139",
pages = "2965--2972",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "12",

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T1 - Partial protection against genital reinfection by immunization of guinea-pigs with isolated outer-membrane proteins of the chlamydial agent of guinea-pig inclusion conjunctivitis

AU - Batteiger, Byron

AU - Rank, R. G.

AU - Bavoil, P. M.

AU - Soderberg, L. S F

PY - 1993

Y1 - 1993

N2 - Because partial protection against reinfection is induced by experimental infection in the guinea-pig model of genital chlamydial infection, we sought to induce immunity by immunization. Female guinea-pigs were immunized subcutaneously with the major outer-membrane protein (MOMP) and the 61 kDa cysteine-rich outer-membrane protein (61 kDa) of the agent of guinea-pig inclusion conjunctivitis (GPIC) eluted from SDS-polyacrylamide gels (SDS-MOMP, SDS-61 kDa). Post-immunization sera and secretions contained antibodies to the SDS-purified proteins at high titre as measured by immunoblotting, whereas enzyme immunoassays (EIA) using whole elementary bodies as antigen showed significantly lower titres (P < 0.001). Likewise, blastogenic responses of peripheral mononuclear cells to GPIC elementary bodies were weak. Animals immunized with SDS-MOMP and SDS-61 kDa were fully susceptible to intravaginal challenge, as were control animals immunized with buffer without protein. Another group of animals were immunized with material prepared by extraction of chlamydial outer-membrane complexes with octyl β-D-glucopyranoside (OGP) and dithiothreitol, which consisted largely of MOMP (OGP-MOMP). In contrast to the SDS-MOMP group, sera and secretions in the OGP-MOMP group showed high titres in EIA, and high titre antibodies to MOMP by immunoblot; however, most animals also had antibodies to 61 kDa, 72 kDa and ca. 84 kDa outer-membrane proteins. OGP-MOMP animals were partially protected against genital challenge as evidenced by low inclusion scores compared to control animals, although duration of infection measured by culture isolation was similar to controls. Immunoblot analysis of sera from immunized animals and from a group of immune animals post-infection was performed using recombinant fusion peptides containing the four variable domains of MOMP. No consistent differences in reaction patterns were observed when sera from protected and non-protected animals were compared. Thus, a highly refined outer-membrane preparation is capable of producing partial immunity to genital infection. Further study is required to determine whether the protection is due to MOMP itself or to other outer-membrane proteins found in small amounts in the OGP-MOMP immunogen. The results suggest the possibility that discontinuous MOMP epitopes could play a role in inducing a protective immune response in the guinea-pig model, a concept that requires further evaluation.

AB - Because partial protection against reinfection is induced by experimental infection in the guinea-pig model of genital chlamydial infection, we sought to induce immunity by immunization. Female guinea-pigs were immunized subcutaneously with the major outer-membrane protein (MOMP) and the 61 kDa cysteine-rich outer-membrane protein (61 kDa) of the agent of guinea-pig inclusion conjunctivitis (GPIC) eluted from SDS-polyacrylamide gels (SDS-MOMP, SDS-61 kDa). Post-immunization sera and secretions contained antibodies to the SDS-purified proteins at high titre as measured by immunoblotting, whereas enzyme immunoassays (EIA) using whole elementary bodies as antigen showed significantly lower titres (P < 0.001). Likewise, blastogenic responses of peripheral mononuclear cells to GPIC elementary bodies were weak. Animals immunized with SDS-MOMP and SDS-61 kDa were fully susceptible to intravaginal challenge, as were control animals immunized with buffer without protein. Another group of animals were immunized with material prepared by extraction of chlamydial outer-membrane complexes with octyl β-D-glucopyranoside (OGP) and dithiothreitol, which consisted largely of MOMP (OGP-MOMP). In contrast to the SDS-MOMP group, sera and secretions in the OGP-MOMP group showed high titres in EIA, and high titre antibodies to MOMP by immunoblot; however, most animals also had antibodies to 61 kDa, 72 kDa and ca. 84 kDa outer-membrane proteins. OGP-MOMP animals were partially protected against genital challenge as evidenced by low inclusion scores compared to control animals, although duration of infection measured by culture isolation was similar to controls. Immunoblot analysis of sera from immunized animals and from a group of immune animals post-infection was performed using recombinant fusion peptides containing the four variable domains of MOMP. No consistent differences in reaction patterns were observed when sera from protected and non-protected animals were compared. Thus, a highly refined outer-membrane preparation is capable of producing partial immunity to genital infection. Further study is required to determine whether the protection is due to MOMP itself or to other outer-membrane proteins found in small amounts in the OGP-MOMP immunogen. The results suggest the possibility that discontinuous MOMP epitopes could play a role in inducing a protective immune response in the guinea-pig model, a concept that requires further evaluation.

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