Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells

Kanji Sugita, Michael Robertson, Yoshihiro Torimoto, Jerome Ritz, Stuart F. Schlossman, Chikao Morimoto

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The CD27 Ag is expressed by the majority of resting T lymphocytes and appears to play a crucial role in T cell activation. We found that some resting peripheral blood NK cells also express CD27. Furthermore, CD27 expression was up-regulated on NK cells stimulated by IL-2. The cytolytic activity of IL-2-activated, but not resting, NK cells was inhibited by an anti-CD27 mAb (anti-1 A4). However, anti-1 A4 did not affect conjugate formation between IL-2-activated NK cells and tumor cell targets. In contrast, anti-1A4 inhibited CD2-mediated calcium mobilization and the serine esterase activity of NK cell granules. These inhibitory effects could be mediated in part by increase in intracellular cAMP levels induced by anti-1 A4. Our results suggest that the CD27 Ag plays an important role in the regulation of activated NK cells.

Original languageEnglish (US)
Pages (from-to)1199-1203
Number of pages5
JournalJournal of Immunology
Volume149
Issue number4
StatePublished - Aug 15 1992
Externally publishedYes

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CD27 Antigens
Natural Killer Cells
Interleukin-2
T-Lymphocytes
Blood Cells
Calcium

ASJC Scopus subject areas

  • Immunology

Cite this

Sugita, K., Robertson, M., Torimoto, Y., Ritz, J., Schlossman, S. F., & Morimoto, C. (1992). Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells. Journal of Immunology, 149(4), 1199-1203.

Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells. / Sugita, Kanji; Robertson, Michael; Torimoto, Yoshihiro; Ritz, Jerome; Schlossman, Stuart F.; Morimoto, Chikao.

In: Journal of Immunology, Vol. 149, No. 4, 15.08.1992, p. 1199-1203.

Research output: Contribution to journalArticle

Sugita, K, Robertson, M, Torimoto, Y, Ritz, J, Schlossman, SF & Morimoto, C 1992, 'Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells', Journal of Immunology, vol. 149, no. 4, pp. 1199-1203.
Sugita K, Robertson M, Torimoto Y, Ritz J, Schlossman SF, Morimoto C. Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells. Journal of Immunology. 1992 Aug 15;149(4):1199-1203.
Sugita, Kanji ; Robertson, Michael ; Torimoto, Yoshihiro ; Ritz, Jerome ; Schlossman, Stuart F. ; Morimoto, Chikao. / Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells. In: Journal of Immunology. 1992 ; Vol. 149, No. 4. pp. 1199-1203.
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