Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells

K. Sugita, M. J. Robertson, Y. Torimoto, J. Ritz, S. F. Schlossman, C. Morimoto

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The CD27 Ag is expressed by the majority of resting T lymphocytes and appears to play a crucial role in T cell activation. We found that some resting peripheral blood NK cells also express CD27. Furthermore, CD27 expression was up-regulated on NK cells stimulated by IL-2. The cytolytic activity of IL-2-activated, but not resting, NK cells was inhibited by an anti-CD27 mAb (anti-1A4). However, anti-1A4 did not affect conjugate formation between IL-2-activated NK cells and tumor cell targets. In contrast, anti-1A4 inhibited CD2-mediated calcium mobilization and the serine esterase activity of NK cell granules. These inhibitory effects could be mediated in part by increase in intracellular cAMP levels induced by anti- 1A4. Our results suggest that the CD27 Ag plays an important role in the regulation of activated NK cells.

Original languageEnglish (US)
Pages (from-to)1199-1203
Number of pages5
JournalJournal of Immunology
Volume149
Issue number4
StatePublished - Jan 1 1992

Fingerprint

CD27 Antigens
Natural Killer Cells
Interleukin-2
T-Lymphocytes
Blood Cells
Calcium

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Sugita, K., Robertson, M. J., Torimoto, Y., Ritz, J., Schlossman, S. F., & Morimoto, C. (1992). Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells. Journal of Immunology, 149(4), 1199-1203.

Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells. / Sugita, K.; Robertson, M. J.; Torimoto, Y.; Ritz, J.; Schlossman, S. F.; Morimoto, C.

In: Journal of Immunology, Vol. 149, No. 4, 01.01.1992, p. 1199-1203.

Research output: Contribution to journalArticle

Sugita, K, Robertson, MJ, Torimoto, Y, Ritz, J, Schlossman, SF & Morimoto, C 1992, 'Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells', Journal of Immunology, vol. 149, no. 4, pp. 1199-1203.
Sugita K, Robertson MJ, Torimoto Y, Ritz J, Schlossman SF, Morimoto C. Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells. Journal of Immunology. 1992 Jan 1;149(4):1199-1203.
Sugita, K. ; Robertson, M. J. ; Torimoto, Y. ; Ritz, J. ; Schlossman, S. F. ; Morimoto, C. / Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells. In: Journal of Immunology. 1992 ; Vol. 149, No. 4. pp. 1199-1203.
@article{e5c9b93ccaeb4574827dd3aa2fb9f5f6,
title = "Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells",
abstract = "The CD27 Ag is expressed by the majority of resting T lymphocytes and appears to play a crucial role in T cell activation. We found that some resting peripheral blood NK cells also express CD27. Furthermore, CD27 expression was up-regulated on NK cells stimulated by IL-2. The cytolytic activity of IL-2-activated, but not resting, NK cells was inhibited by an anti-CD27 mAb (anti-1A4). However, anti-1A4 did not affect conjugate formation between IL-2-activated NK cells and tumor cell targets. In contrast, anti-1A4 inhibited CD2-mediated calcium mobilization and the serine esterase activity of NK cell granules. These inhibitory effects could be mediated in part by increase in intracellular cAMP levels induced by anti- 1A4. Our results suggest that the CD27 Ag plays an important role in the regulation of activated NK cells.",
author = "K. Sugita and Robertson, {M. J.} and Y. Torimoto and J. Ritz and Schlossman, {S. F.} and C. Morimoto",
year = "1992",
month = "1",
day = "1",
language = "English (US)",
volume = "149",
pages = "1199--1203",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells

AU - Sugita, K.

AU - Robertson, M. J.

AU - Torimoto, Y.

AU - Ritz, J.

AU - Schlossman, S. F.

AU - Morimoto, C.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The CD27 Ag is expressed by the majority of resting T lymphocytes and appears to play a crucial role in T cell activation. We found that some resting peripheral blood NK cells also express CD27. Furthermore, CD27 expression was up-regulated on NK cells stimulated by IL-2. The cytolytic activity of IL-2-activated, but not resting, NK cells was inhibited by an anti-CD27 mAb (anti-1A4). However, anti-1A4 did not affect conjugate formation between IL-2-activated NK cells and tumor cell targets. In contrast, anti-1A4 inhibited CD2-mediated calcium mobilization and the serine esterase activity of NK cell granules. These inhibitory effects could be mediated in part by increase in intracellular cAMP levels induced by anti- 1A4. Our results suggest that the CD27 Ag plays an important role in the regulation of activated NK cells.

AB - The CD27 Ag is expressed by the majority of resting T lymphocytes and appears to play a crucial role in T cell activation. We found that some resting peripheral blood NK cells also express CD27. Furthermore, CD27 expression was up-regulated on NK cells stimulated by IL-2. The cytolytic activity of IL-2-activated, but not resting, NK cells was inhibited by an anti-CD27 mAb (anti-1A4). However, anti-1A4 did not affect conjugate formation between IL-2-activated NK cells and tumor cell targets. In contrast, anti-1A4 inhibited CD2-mediated calcium mobilization and the serine esterase activity of NK cell granules. These inhibitory effects could be mediated in part by increase in intracellular cAMP levels induced by anti- 1A4. Our results suggest that the CD27 Ag plays an important role in the regulation of activated NK cells.

UR - http://www.scopus.com/inward/record.url?scp=0026708992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026708992&partnerID=8YFLogxK

M3 - Article

C2 - 1354231

AN - SCOPUS:0026708992

VL - 149

SP - 1199

EP - 1203

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -