Pathogenesis of acute and chronic central nervous system infection with variants of mouse hepatitis virus, strain JHM

Steven Templeton, Stanley Perlman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Infection of mice with variants of mouse hepatitis virus, strain JHM (MHV-JHM), provide models of acute and chronic viral infection of the central nervous system (CNS). Through targeted recombination and reverse genetic manipulation, studies of infection with MHV-JHM variants have identified phenotypic differences and examined the effects of these differences on viral pathogenesis and anti-viral host immune responses. Studies employing recombinant viruses with a modified spike (S) glycoprotein of MHV-JHM have identified the S gene as a major determinant of neurovirulence. However, the association of S gene variation and neurovirulence with host ability to generate anti-viral CD8 T cell responses is not completely clear. Partially protective anti-viral immune responses may result in persistent infection and chronic demyelinating disease characterized by myelin removal from axons of the CNS and associated with dense macrophage/microglial infiltration. Demyelinating disease during MHV-JHM infection is immune-mediated, as mice that lack T lymphocytes fail to develop disease despite succumbing to encephalitis with high levels of infectious virus in the CNS. However, the presence of T lymphocytes or anti-viral antibody can induce disease in infected immunodeficient mice. The mechanisms by which these immune effectors induce demyelination share an ability to activate and recruit macrophages and microglia, thus increasing the putative role of these cells in myelin destruction.

Original languageEnglish (US)
Pages (from-to)160-172
Number of pages13
JournalImmunologic Research
Volume39
Issue number1-3
DOIs
StatePublished - Nov 2007
Externally publishedYes

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Murine hepatitis virus
Central Nervous System Infections
Demyelinating Diseases
Myelin Sheath
Infection
T-Lymphocytes
Central Nervous System Viral Diseases
Central Nervous System
Macrophages
Viral Antibodies
Viruses
Reverse Genetics
Microglia
Encephalitis
Genetic Recombination
Genes
Axons
Anti-Idiotypic Antibodies
Glycoproteins
Chronic Disease

Keywords

  • CNS
  • Demyelination
  • MHV-JHM
  • Neurovirulence
  • Viral infection

ASJC Scopus subject areas

  • Immunology

Cite this

Pathogenesis of acute and chronic central nervous system infection with variants of mouse hepatitis virus, strain JHM. / Templeton, Steven; Perlman, Stanley.

In: Immunologic Research, Vol. 39, No. 1-3, 11.2007, p. 160-172.

Research output: Contribution to journalArticle

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