Patz1 regulates embryonic stem cell identity

Jin Rong Ow, Hui Ma, Angela Jean, Ziyi Goh, Yun Hwa Lee, Yew Mei Chong, Richie Soong, Xin Yuan Fu, Henry Yang, Qiang Wu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Embryonic stem cells (ESCs) derived from the inner cell mass (ICM) of blastocysts are pluripotent. Pluripotency is maintained by a transcriptional network in which Oct4 and Nanog are master regulators. Notably, several zinc finger transcription factors have important roles in this network. Patz1, a BTB/POZ-domain-containing zinc finger protein, is expressed at higher levels in the ICM relative to the trophectoderm. However, its function in pluripotency has been poorly studied. Here, we show that Patz1 is an important regulator of pluripotency in ESCs. Patz1 RNAi, chromatin immunoprecipitation (ChIP), and reporter assays indicate that Patz1 directly regulates Pou5f1 and Nanog. Global transcriptome changes upon Patz1 knockdown largely involve upregulation of apoptotic genes and downregulation of cell cycle and cellular metabolism genes. Patz1 ChIP sequencing further identified more than 5,000 binding sites of Patz1 in mouse genome, from which two binding motifs were extracted. Further, gene ontology analysis of genes associated with the binding sites displays enrichment for proximity to developmental genes. In addition, embryoid body assays suggest that Patz1 represses developmental genes. Together, these results propose that Patz1 is important for ESC pluripotency.

Original languageEnglish (US)
Pages (from-to)1062-1073
Number of pages12
JournalStem Cells and Development
Volume23
Issue number10
DOIs
StatePublished - May 15 2014
Externally publishedYes

Fingerprint

Embryonic Stem Cells
Developmental Genes
Chromatin Immunoprecipitation
Zinc Fingers
Blastocyst Inner Cell Mass
Binding Sites
Embryoid Bodies
cdc Genes
Gene Ontology
Gene Regulatory Networks
RNA Interference
Transcriptome
Genes
Transcription Factors
Up-Regulation
Down-Regulation
Genome
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology
  • Medicine(all)

Cite this

Ow, J. R., Ma, H., Jean, A., Goh, Z., Lee, Y. H., Chong, Y. M., ... Wu, Q. (2014). Patz1 regulates embryonic stem cell identity. Stem Cells and Development, 23(10), 1062-1073. https://doi.org/10.1089/scd.2013.0430

Patz1 regulates embryonic stem cell identity. / Ow, Jin Rong; Ma, Hui; Jean, Angela; Goh, Ziyi; Lee, Yun Hwa; Chong, Yew Mei; Soong, Richie; Fu, Xin Yuan; Yang, Henry; Wu, Qiang.

In: Stem Cells and Development, Vol. 23, No. 10, 15.05.2014, p. 1062-1073.

Research output: Contribution to journalArticle

Ow, JR, Ma, H, Jean, A, Goh, Z, Lee, YH, Chong, YM, Soong, R, Fu, XY, Yang, H & Wu, Q 2014, 'Patz1 regulates embryonic stem cell identity', Stem Cells and Development, vol. 23, no. 10, pp. 1062-1073. https://doi.org/10.1089/scd.2013.0430
Ow JR, Ma H, Jean A, Goh Z, Lee YH, Chong YM et al. Patz1 regulates embryonic stem cell identity. Stem Cells and Development. 2014 May 15;23(10):1062-1073. https://doi.org/10.1089/scd.2013.0430
Ow, Jin Rong ; Ma, Hui ; Jean, Angela ; Goh, Ziyi ; Lee, Yun Hwa ; Chong, Yew Mei ; Soong, Richie ; Fu, Xin Yuan ; Yang, Henry ; Wu, Qiang. / Patz1 regulates embryonic stem cell identity. In: Stem Cells and Development. 2014 ; Vol. 23, No. 10. pp. 1062-1073.
@article{52110610ebbb478caf6016787202c5fc,
title = "Patz1 regulates embryonic stem cell identity",
abstract = "Embryonic stem cells (ESCs) derived from the inner cell mass (ICM) of blastocysts are pluripotent. Pluripotency is maintained by a transcriptional network in which Oct4 and Nanog are master regulators. Notably, several zinc finger transcription factors have important roles in this network. Patz1, a BTB/POZ-domain-containing zinc finger protein, is expressed at higher levels in the ICM relative to the trophectoderm. However, its function in pluripotency has been poorly studied. Here, we show that Patz1 is an important regulator of pluripotency in ESCs. Patz1 RNAi, chromatin immunoprecipitation (ChIP), and reporter assays indicate that Patz1 directly regulates Pou5f1 and Nanog. Global transcriptome changes upon Patz1 knockdown largely involve upregulation of apoptotic genes and downregulation of cell cycle and cellular metabolism genes. Patz1 ChIP sequencing further identified more than 5,000 binding sites of Patz1 in mouse genome, from which two binding motifs were extracted. Further, gene ontology analysis of genes associated with the binding sites displays enrichment for proximity to developmental genes. In addition, embryoid body assays suggest that Patz1 represses developmental genes. Together, these results propose that Patz1 is important for ESC pluripotency.",
author = "Ow, {Jin Rong} and Hui Ma and Angela Jean and Ziyi Goh and Lee, {Yun Hwa} and Chong, {Yew Mei} and Richie Soong and Fu, {Xin Yuan} and Henry Yang and Qiang Wu",
year = "2014",
month = "5",
day = "15",
doi = "10.1089/scd.2013.0430",
language = "English (US)",
volume = "23",
pages = "1062--1073",
journal = "Stem Cells and Development",
issn = "1547-3287",
publisher = "Mary Ann Liebert Inc.",
number = "10",

}

TY - JOUR

T1 - Patz1 regulates embryonic stem cell identity

AU - Ow, Jin Rong

AU - Ma, Hui

AU - Jean, Angela

AU - Goh, Ziyi

AU - Lee, Yun Hwa

AU - Chong, Yew Mei

AU - Soong, Richie

AU - Fu, Xin Yuan

AU - Yang, Henry

AU - Wu, Qiang

PY - 2014/5/15

Y1 - 2014/5/15

N2 - Embryonic stem cells (ESCs) derived from the inner cell mass (ICM) of blastocysts are pluripotent. Pluripotency is maintained by a transcriptional network in which Oct4 and Nanog are master regulators. Notably, several zinc finger transcription factors have important roles in this network. Patz1, a BTB/POZ-domain-containing zinc finger protein, is expressed at higher levels in the ICM relative to the trophectoderm. However, its function in pluripotency has been poorly studied. Here, we show that Patz1 is an important regulator of pluripotency in ESCs. Patz1 RNAi, chromatin immunoprecipitation (ChIP), and reporter assays indicate that Patz1 directly regulates Pou5f1 and Nanog. Global transcriptome changes upon Patz1 knockdown largely involve upregulation of apoptotic genes and downregulation of cell cycle and cellular metabolism genes. Patz1 ChIP sequencing further identified more than 5,000 binding sites of Patz1 in mouse genome, from which two binding motifs were extracted. Further, gene ontology analysis of genes associated with the binding sites displays enrichment for proximity to developmental genes. In addition, embryoid body assays suggest that Patz1 represses developmental genes. Together, these results propose that Patz1 is important for ESC pluripotency.

AB - Embryonic stem cells (ESCs) derived from the inner cell mass (ICM) of blastocysts are pluripotent. Pluripotency is maintained by a transcriptional network in which Oct4 and Nanog are master regulators. Notably, several zinc finger transcription factors have important roles in this network. Patz1, a BTB/POZ-domain-containing zinc finger protein, is expressed at higher levels in the ICM relative to the trophectoderm. However, its function in pluripotency has been poorly studied. Here, we show that Patz1 is an important regulator of pluripotency in ESCs. Patz1 RNAi, chromatin immunoprecipitation (ChIP), and reporter assays indicate that Patz1 directly regulates Pou5f1 and Nanog. Global transcriptome changes upon Patz1 knockdown largely involve upregulation of apoptotic genes and downregulation of cell cycle and cellular metabolism genes. Patz1 ChIP sequencing further identified more than 5,000 binding sites of Patz1 in mouse genome, from which two binding motifs were extracted. Further, gene ontology analysis of genes associated with the binding sites displays enrichment for proximity to developmental genes. In addition, embryoid body assays suggest that Patz1 represses developmental genes. Together, these results propose that Patz1 is important for ESC pluripotency.

UR - http://www.scopus.com/inward/record.url?scp=84900459544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900459544&partnerID=8YFLogxK

U2 - 10.1089/scd.2013.0430

DO - 10.1089/scd.2013.0430

M3 - Article

VL - 23

SP - 1062

EP - 1073

JO - Stem Cells and Development

JF - Stem Cells and Development

SN - 1547-3287

IS - 10

ER -