Patz1 regulates embryonic stem cell identity

Jin Rong Ow, Hui Ma, Angela Jean, Ziyi Goh, Yun Hwa Lee, Yew Mei Chong, Richie Soong, Xin Yuan Fu, Henry Yang, Qiang Wu

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Embryonic stem cells (ESCs) derived from the inner cell mass (ICM) of blastocysts are pluripotent. Pluripotency is maintained by a transcriptional network in which Oct4 and Nanog are master regulators. Notably, several zinc finger transcription factors have important roles in this network. Patz1, a BTB/POZ-domain-containing zinc finger protein, is expressed at higher levels in the ICM relative to the trophectoderm. However, its function in pluripotency has been poorly studied. Here, we show that Patz1 is an important regulator of pluripotency in ESCs. Patz1 RNAi, chromatin immunoprecipitation (ChIP), and reporter assays indicate that Patz1 directly regulates Pou5f1 and Nanog. Global transcriptome changes upon Patz1 knockdown largely involve upregulation of apoptotic genes and downregulation of cell cycle and cellular metabolism genes. Patz1 ChIP sequencing further identified more than 5,000 binding sites of Patz1 in mouse genome, from which two binding motifs were extracted. Further, gene ontology analysis of genes associated with the binding sites displays enrichment for proximity to developmental genes. In addition, embryoid body assays suggest that Patz1 represses developmental genes. Together, these results propose that Patz1 is important for ESC pluripotency.

Original languageEnglish (US)
Pages (from-to)1062-1073
Number of pages12
JournalStem Cells and Development
Volume23
Issue number10
DOIs
StatePublished - May 15 2014

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

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    Ow, J. R., Ma, H., Jean, A., Goh, Z., Lee, Y. H., Chong, Y. M., Soong, R., Fu, X. Y., Yang, H., & Wu, Q. (2014). Patz1 regulates embryonic stem cell identity. Stem Cells and Development, 23(10), 1062-1073. https://doi.org/10.1089/scd.2013.0430