PAX8 is expressed in the majority of renal epithelial neoplasms: An immunohistochemical study of 223 cases using a mouse monoclonal antibody

Yingchuan Hu, Arndt Hartmann, Christine Stoehr, Shaobo Zhang, Mingsheng Wang, David Tacha, Rodolfo Montironi, Antonio Lopez-Beltran, Liang Cheng

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Aims: PAX8 is a cell lineage-specific transcription factor which plays a crucial role in the organogenesis of the kidney, thyroid gland and Müllerian duct. A previous study showed that PAX8 is a specific and sensitive marker for both renal and ovarian carcinomas. The purpose of this study is to investigate PAX8 expression using a new monoclonal PAX8 antibody in a larger number of renal epithelial neoplasms including clear cell renal cell carcinoma, papillary renal cell carcinoma, chromophobe renal cell carcinoma and renal oncocytoma. Methods: PAX8 immunohistochemical staining was performed on tissue microarrays containing 84 cases of clear cell renal cell carcinoma, 66 cases of chromophobe renal cell carcinoma, 57 cases of papillary renal cell carcinoma and 16 cases of renal oncocytoma. Results: PAX8 expression was detected in 93% (78/84) of cases of clear cell renal cell carcinoma, 80% (53/66) of cases of chromophobe renal cell carcinoma, 95% (54/ 57) of cases of papillary renal cell carcinoma and 94% (15/16) of cases of renal oncocytoma. Conclusions: PAX8 is expressed in the majority of renal epithelial neoplasms including renal cell carcinomas and oncocytomas and the monoclonal PAX8 antibody is more sensitive than polyclonal antibody to detect chromophobe renal cell carcinoma. These results showed that PAX8 is a valuable marker for nephric neoplasms.

Original languageEnglish
Pages (from-to)254-256
Number of pages3
JournalJournal of Clinical Pathology
Volume65
Issue number3
DOIs
StatePublished - Mar 2012

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Glandular and Epithelial Neoplasms
Kidney Neoplasms
Renal Cell Carcinoma
Monoclonal Antibodies
Kidney
Organogenesis
Cell Lineage
Thyroid Gland
Transcription Factors
Staining and Labeling
Carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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PAX8 is expressed in the majority of renal epithelial neoplasms : An immunohistochemical study of 223 cases using a mouse monoclonal antibody. / Hu, Yingchuan; Hartmann, Arndt; Stoehr, Christine; Zhang, Shaobo; Wang, Mingsheng; Tacha, David; Montironi, Rodolfo; Lopez-Beltran, Antonio; Cheng, Liang.

In: Journal of Clinical Pathology, Vol. 65, No. 3, 03.2012, p. 254-256.

Research output: Contribution to journalArticle

Hu, Yingchuan ; Hartmann, Arndt ; Stoehr, Christine ; Zhang, Shaobo ; Wang, Mingsheng ; Tacha, David ; Montironi, Rodolfo ; Lopez-Beltran, Antonio ; Cheng, Liang. / PAX8 is expressed in the majority of renal epithelial neoplasms : An immunohistochemical study of 223 cases using a mouse monoclonal antibody. In: Journal of Clinical Pathology. 2012 ; Vol. 65, No. 3. pp. 254-256.
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abstract = "Aims: PAX8 is a cell lineage-specific transcription factor which plays a crucial role in the organogenesis of the kidney, thyroid gland and M{\"u}llerian duct. A previous study showed that PAX8 is a specific and sensitive marker for both renal and ovarian carcinomas. The purpose of this study is to investigate PAX8 expression using a new monoclonal PAX8 antibody in a larger number of renal epithelial neoplasms including clear cell renal cell carcinoma, papillary renal cell carcinoma, chromophobe renal cell carcinoma and renal oncocytoma. Methods: PAX8 immunohistochemical staining was performed on tissue microarrays containing 84 cases of clear cell renal cell carcinoma, 66 cases of chromophobe renal cell carcinoma, 57 cases of papillary renal cell carcinoma and 16 cases of renal oncocytoma. Results: PAX8 expression was detected in 93{\%} (78/84) of cases of clear cell renal cell carcinoma, 80{\%} (53/66) of cases of chromophobe renal cell carcinoma, 95{\%} (54/ 57) of cases of papillary renal cell carcinoma and 94{\%} (15/16) of cases of renal oncocytoma. Conclusions: PAX8 is expressed in the majority of renal epithelial neoplasms including renal cell carcinomas and oncocytomas and the monoclonal PAX8 antibody is more sensitive than polyclonal antibody to detect chromophobe renal cell carcinoma. These results showed that PAX8 is a valuable marker for nephric neoplasms.",
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AU - Zhang, Shaobo

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AU - Tacha, David

AU - Montironi, Rodolfo

AU - Lopez-Beltran, Antonio

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