Pbx1 functions in distinct regulatory networks to pattern the great arteries and cardiac outflow tract

Ching-Pin Chang, Kryn Stankunas, Ching Shang, Shih Chu Kao, Karen Y. Twu, Michael L. Cleary

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The patterning of the cardiovascular system into systemic and pulmonic circulations is a complex morphogenetic process, the failure of which results in clinically important congenital defects. This process involves extensive vascular remodeling and coordinated division of the cardiac outflow tract (OFT). We demonstrate that the homeodomain transcription factor Pbx1 orchestrates separate transcriptional pathways to control great-artery patterning and cardiac OFT septation in mice. Pbx1-null embryos display anomalous great arteries owing to a failure to establish the initial complement of branchial arch arteries in the caudal pharyngeal region. Pbx1 deficiency also results in the failure of cardiac OFT septation. Pbx1-null embryos lose a transient burst of Pax3 expression in premigratory cardiac neural crest cells (NCCs) that ultimately specifies cardiac NCC function for OFT development, but does not regulate NCC migration to the heart. We show that Pbx1 directly activates Pax3, leading to repression of its target gene Msx2 in NCCs. Compound Msx2/Pbx1-null embryos display significant rescue of cardiac septation, demonstrating that disruption of this Pbx1-Pax3-Msx2 regulatory pathway partially underlies the OFT defects in Pbx1-null mice. Conversely, the great-artery anomalies of compound Msx2/Pbx1-null embryos remain within the same spectrum as those of Pbx1-null embryos. Thus, Pbx1 makes a crucial contribution to distinct regulatory pathways in cardiovascular development.

Original languageEnglish (US)
Pages (from-to)3577-3586
Number of pages10
JournalDevelopment (Cambridge)
Volume135
Issue number21
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Neural Crest
Embryonic Structures
Arteries
Branchial Region
Cardiovascular System
Cell Movement
Transcription Factors
Heart Failure
Lung
Genes

Keywords

  • Heart development
  • Hox
  • Mouse
  • Msx2
  • Pax3
  • Pbx
  • Vascular patterning

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Chang, C-P., Stankunas, K., Shang, C., Kao, S. C., Twu, K. Y., & Cleary, M. L. (2008). Pbx1 functions in distinct regulatory networks to pattern the great arteries and cardiac outflow tract. Development (Cambridge), 135(21), 3577-3586. https://doi.org/10.1242/dev.022350

Pbx1 functions in distinct regulatory networks to pattern the great arteries and cardiac outflow tract. / Chang, Ching-Pin; Stankunas, Kryn; Shang, Ching; Kao, Shih Chu; Twu, Karen Y.; Cleary, Michael L.

In: Development (Cambridge), Vol. 135, No. 21, 2008, p. 3577-3586.

Research output: Contribution to journalArticle

Chang, C-P, Stankunas, K, Shang, C, Kao, SC, Twu, KY & Cleary, ML 2008, 'Pbx1 functions in distinct regulatory networks to pattern the great arteries and cardiac outflow tract', Development (Cambridge), vol. 135, no. 21, pp. 3577-3586. https://doi.org/10.1242/dev.022350
Chang, Ching-Pin ; Stankunas, Kryn ; Shang, Ching ; Kao, Shih Chu ; Twu, Karen Y. ; Cleary, Michael L. / Pbx1 functions in distinct regulatory networks to pattern the great arteries and cardiac outflow tract. In: Development (Cambridge). 2008 ; Vol. 135, No. 21. pp. 3577-3586.
@article{12f69c76ff3448118fe2662ef6b9177b,
title = "Pbx1 functions in distinct regulatory networks to pattern the great arteries and cardiac outflow tract",
abstract = "The patterning of the cardiovascular system into systemic and pulmonic circulations is a complex morphogenetic process, the failure of which results in clinically important congenital defects. This process involves extensive vascular remodeling and coordinated division of the cardiac outflow tract (OFT). We demonstrate that the homeodomain transcription factor Pbx1 orchestrates separate transcriptional pathways to control great-artery patterning and cardiac OFT septation in mice. Pbx1-null embryos display anomalous great arteries owing to a failure to establish the initial complement of branchial arch arteries in the caudal pharyngeal region. Pbx1 deficiency also results in the failure of cardiac OFT septation. Pbx1-null embryos lose a transient burst of Pax3 expression in premigratory cardiac neural crest cells (NCCs) that ultimately specifies cardiac NCC function for OFT development, but does not regulate NCC migration to the heart. We show that Pbx1 directly activates Pax3, leading to repression of its target gene Msx2 in NCCs. Compound Msx2/Pbx1-null embryos display significant rescue of cardiac septation, demonstrating that disruption of this Pbx1-Pax3-Msx2 regulatory pathway partially underlies the OFT defects in Pbx1-null mice. Conversely, the great-artery anomalies of compound Msx2/Pbx1-null embryos remain within the same spectrum as those of Pbx1-null embryos. Thus, Pbx1 makes a crucial contribution to distinct regulatory pathways in cardiovascular development.",
keywords = "Heart development, Hox, Mouse, Msx2, Pax3, Pbx, Vascular patterning",
author = "Ching-Pin Chang and Kryn Stankunas and Ching Shang and Kao, {Shih Chu} and Twu, {Karen Y.} and Cleary, {Michael L.}",
year = "2008",
doi = "10.1242/dev.022350",
language = "English (US)",
volume = "135",
pages = "3577--3586",
journal = "Development",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "21",

}

TY - JOUR

T1 - Pbx1 functions in distinct regulatory networks to pattern the great arteries and cardiac outflow tract

AU - Chang, Ching-Pin

AU - Stankunas, Kryn

AU - Shang, Ching

AU - Kao, Shih Chu

AU - Twu, Karen Y.

AU - Cleary, Michael L.

PY - 2008

Y1 - 2008

N2 - The patterning of the cardiovascular system into systemic and pulmonic circulations is a complex morphogenetic process, the failure of which results in clinically important congenital defects. This process involves extensive vascular remodeling and coordinated division of the cardiac outflow tract (OFT). We demonstrate that the homeodomain transcription factor Pbx1 orchestrates separate transcriptional pathways to control great-artery patterning and cardiac OFT septation in mice. Pbx1-null embryos display anomalous great arteries owing to a failure to establish the initial complement of branchial arch arteries in the caudal pharyngeal region. Pbx1 deficiency also results in the failure of cardiac OFT septation. Pbx1-null embryos lose a transient burst of Pax3 expression in premigratory cardiac neural crest cells (NCCs) that ultimately specifies cardiac NCC function for OFT development, but does not regulate NCC migration to the heart. We show that Pbx1 directly activates Pax3, leading to repression of its target gene Msx2 in NCCs. Compound Msx2/Pbx1-null embryos display significant rescue of cardiac septation, demonstrating that disruption of this Pbx1-Pax3-Msx2 regulatory pathway partially underlies the OFT defects in Pbx1-null mice. Conversely, the great-artery anomalies of compound Msx2/Pbx1-null embryos remain within the same spectrum as those of Pbx1-null embryos. Thus, Pbx1 makes a crucial contribution to distinct regulatory pathways in cardiovascular development.

AB - The patterning of the cardiovascular system into systemic and pulmonic circulations is a complex morphogenetic process, the failure of which results in clinically important congenital defects. This process involves extensive vascular remodeling and coordinated division of the cardiac outflow tract (OFT). We demonstrate that the homeodomain transcription factor Pbx1 orchestrates separate transcriptional pathways to control great-artery patterning and cardiac OFT septation in mice. Pbx1-null embryos display anomalous great arteries owing to a failure to establish the initial complement of branchial arch arteries in the caudal pharyngeal region. Pbx1 deficiency also results in the failure of cardiac OFT septation. Pbx1-null embryos lose a transient burst of Pax3 expression in premigratory cardiac neural crest cells (NCCs) that ultimately specifies cardiac NCC function for OFT development, but does not regulate NCC migration to the heart. We show that Pbx1 directly activates Pax3, leading to repression of its target gene Msx2 in NCCs. Compound Msx2/Pbx1-null embryos display significant rescue of cardiac septation, demonstrating that disruption of this Pbx1-Pax3-Msx2 regulatory pathway partially underlies the OFT defects in Pbx1-null mice. Conversely, the great-artery anomalies of compound Msx2/Pbx1-null embryos remain within the same spectrum as those of Pbx1-null embryos. Thus, Pbx1 makes a crucial contribution to distinct regulatory pathways in cardiovascular development.

KW - Heart development

KW - Hox

KW - Mouse

KW - Msx2

KW - Pax3

KW - Pbx

KW - Vascular patterning

UR - http://www.scopus.com/inward/record.url?scp=58149346044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149346044&partnerID=8YFLogxK

U2 - 10.1242/dev.022350

DO - 10.1242/dev.022350

M3 - Article

VL - 135

SP - 3577

EP - 3586

JO - Development

JF - Development

SN - 0950-1991

IS - 21

ER -