P‐component of amyloid. Isolation from human serum by affinity chromatography

Merrill D. Benson, Martha Skinner, Tsuranobu Shirahama, Alan S. Cohen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Human amyloid P-component was isolated from plasma by affinity chromatography utilizing antiserum to tissue P-component coupled to Sepharose 4B. Characterization and comparison of the isolated P-component proteins from tissue and plasma demonstrated immunologic identity and identical RF values of polyacrylamide disk gel electrophoresis. Amino acid analyses of P-components from two individual plasmas were comparable, but some variations from tissue P-component were noted. Attempts to perform amino acid sequencing on the plasma protein were unsuccessful. Electron microscopic studies revealed that the plasma P-component had a pentagonal ultrastructure identical to the tissue P-component. These studies compared similarities of tissue and plasma P-components and revealed the unique pentagonal ultrastructure of the plasma protein.

Original languageEnglish (US)
Pages (from-to)749-754
Number of pages6
JournalArthritis & Rheumatism
Volume19
Issue number4
DOIs
StatePublished - Jan 1 1976

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Serum Amyloid P-Component
Affinity Chromatography
Blood Proteins
Serum
Disc Electrophoresis
Protein Sequence Analysis
Sepharose
Immune Sera
Polyacrylamide Gel Electrophoresis
Electrons
Amino Acids

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

P‐component of amyloid. Isolation from human serum by affinity chromatography. / Benson, Merrill D.; Skinner, Martha; Shirahama, Tsuranobu; Cohen, Alan S.

In: Arthritis & Rheumatism, Vol. 19, No. 4, 01.01.1976, p. 749-754.

Research output: Contribution to journalArticle

Benson, M, Skinner, M, Shirahama, T & Cohen, AS 1976, 'P-component of amyloid. Isolation from human serum by affinity chromatography.', Arthritis and Rheumatism, vol. 19, no. 4, pp. 749-754.
Benson, Merrill ; Skinner, M. ; Shirahama, T. ; Cohen, A. S. / P-component of amyloid. Isolation from human serum by affinity chromatography. In: Arthritis and Rheumatism. 1976 ; Vol. 19, No. 4. pp. 749-754.
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