PCR cloning and sequence of two cDNAs encoding the alpha and beta subunits of rabbit casein kinase-II

Shalley K. Gupta, Jai Pal Singh

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Polymerase chain reaction (PCR) amplification of total RNA from vascular smooth muscle cells (VSMC) was used to clone and sequence full-length cDNAs encoding the a and β subunits of rabbit casein kinase-II (CK-II). A strong homology and evolutionary conservation was found in both the nucleotide (nt) and deduced amino acid sequences of CK-II from various species, with up to 100% identity among vertebrate homologues and 88% and 64% identity on the nt level with Drosophila melanogaster and yeast, respectively, compared with rabbit CK-II. The cloning and expression of rabbit CK-II will allow us to generate antibody and cDNA probes to investigate the role of CK-II in VSMC growth, migration, and phenotypic transformation during the pathogenesis of vascular disease.

Original languageEnglish (US)
Pages (from-to)287-290
Number of pages4
JournalGene
Volume124
Issue number2
DOIs
StatePublished - Feb 28 1993

Keywords

  • Serine/threonine kinase
  • signal transduction
  • vascular disease
  • vascular smooth muscle cell

ASJC Scopus subject areas

  • Genetics

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