Peptidase inhibitors improve recovery of substance P and calcitonin gene-related peptide release from rat spinal cord slices

Joanne J. Chen, Lisa A. Barber, Julia Dymshitz, Michael Vasko

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The purpose of present study was to determine whether peptidase activity affects the release of substance P (SP) and calcitonin gene-related peptide (CGRP) from spinal cord slices. When slices were exposed to various inhibitors of endopeptidase 24.11, the resting and capsaicin-stimulated release of SP were less than 0.04% and 0.20% total content per minute, respectively. Resting CGRP release was approximately 0.10% and stimulated release was 0.40%. The combination of 20 μM bacitracin, 100 μM phenylalanylalanine (Phe-Ala), and 50 μM p-chloromercuriphenylsulfonic acid (PCMS) significantly increased both resting and stimulated release of SP and CGRP at least two- or threefold. Doubling the concentration of PCMS and Phe-Ala did not further improve peptide release. These results demonstrate that recovery of peptides released from spinal cord slices is dependent in part on activity of multiple peptidases in the tissues.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalPeptides
Volume17
Issue number1
DOIs
StatePublished - 1996

Fingerprint

Calcitonin Gene-Related Peptide
phenylalanylalanine
Substance P
Protease Inhibitors
Rats
Spinal Cord
Recovery
Peptide Hydrolases
Bacitracin
Neprilysin
Peptides
Acids
Capsaicin
Tissue

Keywords

  • Calcitonin gene-related peptide
  • Capsaicin
  • Peptidase inhibitors
  • Release
  • Spinal cord slices
  • Substance P

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Peptidase inhibitors improve recovery of substance P and calcitonin gene-related peptide release from rat spinal cord slices. / Chen, Joanne J.; Barber, Lisa A.; Dymshitz, Julia; Vasko, Michael.

In: Peptides, Vol. 17, No. 1, 1996, p. 31-37.

Research output: Contribution to journalArticle

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