Peptide transporter function and prolidase activities in caco-2 cells: A lack of coordinated expression

Ming Hu, Lixing Zheng, Jiyue Chen, Lijuan Liu, Yiqi Li, Anne H. Dantzig, Robert E. Stratford

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Peptide transport and prolidase activities were measured to determine whether the expression of these two components of protein nutrition are coordinately regulated; i.e., whether an increase in the peptide transporter function will necessarily lead to a higher prolidase activity, or vice versa. The results indicated that peptide transporter function and prolidase activity respond differently to cell differentiation and feeding schedules. The results also indicated that peptide transport and prolidase activities were different in two Caco-2 cell 'clones' with S-K cells transported peptides at higher rates but had lower total prolidase activities, when compared to ATCC cells. These results suggest that the expression of the peptide transporter function and prolidase activity is not coordinated. In addition, both the transporter and the prolidase activities affected the overall transport of Phe when given as the dipeptide Phe-Pro, supporting the notion that intestinal absorption of peptides is an essential component of amino acid absorption. In conclusion, the evidence suggests that the peptide transporter function and prolidase activity are not coordinately expressed by the human intestinal Caco-2 cells.

Original languageEnglish (US)
Pages (from-to)291-300
Number of pages10
JournalJournal of Drug Targeting
Volume3
Issue number4
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Keywords

  • Dipeptide
  • Intracellular metabolism
  • Peptidase
  • Peptide transporter
  • Prolidase
  • Transcellular transport

ASJC Scopus subject areas

  • Pharmaceutical Science

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