Peptidoglycan recognition protein 3 and Nod2 synergistically protect mice from dextran sodium sulfate-induced colitis

Xuefang Jing, Fareeha Zulfiqar, Shin Yong Park, Gabriel Núñez, Roman Dziarski, Dipika Gupta

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Aberrant immune response and changes in the gut microflora are the main causes of inflammatory bowel disease (IBD). Peptidoglycan recognition proteins (Pglyrp1, Pglyrp2, Pglyrp3, and Pglyrp4) are bactericidal innate immunity proteins that maintain normal gut microbiome, protect against experimental colitis, and are associated with IBD in humans. Nucleotide-binding oligomerization domain 2 (Nod2) is an intracellular bacterial sensor and may be required for maintaining normal gut microbiome. Mutations in Nod2 are strongly associated with Crohn's disease, but the causative mechanism is not understood, and the role of Nod2 in ulcerative colitis is not known. Because IBD is likely caused by variable multiple mutations in different individuals, in this study, we examined the combined role of Pglyrp3 and Nod2 in the development of experimental colitis in mice. We demonstrate that a combined deficiency of Pglyrp3 and Nod2 results in higher sensitivity to dextran sodium sulfate-induced colitis compared with a single deficiency. Pglyrp3<sup>-/-</sup>Nod2<sup>-/-</sup>mice had decreased survival and higher loss of body weight, increased intestinal bleeding, higher apoptosis of colonic mucosa, elevated expression of cytokines and chemokines, altered gut microbiome, and increased levels of ATP in the colon. Increased sensitivity to dextran sodium sulfate-induced colitis in Pglyrp3<sup>-/-</sup>Nod2<sup>-/-</sup>mice depended on increased apoptosis of intestinal epithelium, changed gut microflora, and elevated ATP. Pglyrp3 deficiency contributed colitis-predisposing intestinal microflora and increased intestinal ATP, whereas Nod2 deficiency contributed higher apoptosis and responsiveness to increased level of ATP. In summary, Pglyrp3 and Nod2 are both required for maintaining gut homeostasis and protection against colitis, but their protective mechanisms differ.

Original languageEnglish
Pages (from-to)3055-3069
Number of pages15
JournalJournal of Immunology
Volume193
Issue number6
DOIs
StatePublished - Sep 15 2014

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Dextran Sulfate
Colitis
Nucleotides
Adenosine Triphosphate
Inflammatory Bowel Diseases
Apoptosis
peptidoglycan recognition protein
Mutation
Intestinal Mucosa
Ulcerative Colitis
Chemokines
Innate Immunity
Crohn Disease
Gastrointestinal Microbiome
Colon
Mucous Membrane
Homeostasis
Body Weight
Hemorrhage
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Peptidoglycan recognition protein 3 and Nod2 synergistically protect mice from dextran sodium sulfate-induced colitis. / Jing, Xuefang; Zulfiqar, Fareeha; Park, Shin Yong; Núñez, Gabriel; Dziarski, Roman; Gupta, Dipika.

In: Journal of Immunology, Vol. 193, No. 6, 15.09.2014, p. 3055-3069.

Research output: Contribution to journalArticle

Jing, Xuefang ; Zulfiqar, Fareeha ; Park, Shin Yong ; Núñez, Gabriel ; Dziarski, Roman ; Gupta, Dipika. / Peptidoglycan recognition protein 3 and Nod2 synergistically protect mice from dextran sodium sulfate-induced colitis. In: Journal of Immunology. 2014 ; Vol. 193, No. 6. pp. 3055-3069.
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