Performance characteristics of the platelia Aspergillus enzyme immunoassay for detection of Aspergillus galactomannan antigen in bronchoalveolar lavage fluid

S. Husain, C. J. Clancy, M. H. Nguyen, S. Swartzentruber, H. Leather, A. M. LeMonte, M. M. Durkin, K. S. Knox, C. A. Hage, C. Bentsen, N. Singh, J. R. Wingard, L. J. Wheat

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

We have evaluated the Platelia Aspergillus enzyme immunoassay for detection of galactomannan in bronchoalveolar lavage (BAL) specimens in solid organ transplant patients with aspergillosis. The precision and reproducibility in serum or BAL to which galactomannan was added were similar. Sensitivity was 81.8% in patients with aspergillosis, and specificity was 95.8% in lung transplant patients who underwent BAL for surveillance for infection or rejection. Among transplant controls, positive results were more common in patients (i) who underwent diagnostic BAL performed for evaluation of symptoms or chest computed tomographic abnormalities, (ii) who had undergone lung transplantation, or (iii) who were colonized with Aspergillus. Galactomannan testing in BAL is useful for diagnosis of aspergillosis in transplant patients. The significance of positive results in patients without confirmed aspergillosis requires further evaluation.

Original languageEnglish (US)
Pages (from-to)1760-1763
Number of pages4
JournalClinical and Vaccine Immunology
Volume15
Issue number12
DOIs
StatePublished - Dec 2008

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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