Performance of the cobas MRSA/SA Test for Simultaneous Detection of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus from Nasal Swabs

Lance R. Peterson, Christopher W. Woods, Thomas Davis, Zi Xuam Wang, Stephen A. Young, John C. Osiecki, Michael A. Lewinski, Oliver Liesenfeld

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives Health care-associated methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus (SA) infections are continuing problems. Rapidly determining the MRSA colonization status of a patient facilitates practice to reduce spread of MRSA clinical disease. Sensitive detection of all SA prior to surgery, followed by decolonization, can significantly reduce postoperative infection from this pathogen. Our goal was to validate a new automated assay for this testing. Methods We compared performance of the cobas MRSA/SA Test on the cobas 4800 System to direct and enriched chromogenic culture using nasal swabs collected from patients at six United States sites. Results Compared to direct and enriched culture, the sensitivity for MRSA and SA was 93.1% and 93.9%, and the specificity was 97.5% and 94.2%, respectively. After discrepancy analysis, the sensitivity for MRSA and SA was 97.1% and 98.6%, and the specificity was 98.3% and 95.5%, respectively. Compared to direct culture, sensitivity for detecting any SA was 99.6%. Conclusions The cobas MRSA/SA Test is an effective tool to simultaneously perform surveillance testing for nasal colonization of both MRSA and MSSA.

Original languageEnglish (US)
Pages (from-to)119-127
Number of pages9
JournalAmerican Journal of Clinical Pathology
Volume148
Issue number2
DOIs
StatePublished - Aug 1 2017

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Methicillin
Methicillin-Resistant Staphylococcus aureus
Nose
Staphylococcus aureus
Infection
Delivery of Health Care

Keywords

  • Infection control
  • MRSA
  • Real-time PCR
  • Staphylococcus aureus
  • Surgical site infection

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Performance of the cobas MRSA/SA Test for Simultaneous Detection of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus from Nasal Swabs. / Peterson, Lance R.; Woods, Christopher W.; Davis, Thomas; Wang, Zi Xuam; Young, Stephen A.; Osiecki, John C.; Lewinski, Michael A.; Liesenfeld, Oliver.

In: American Journal of Clinical Pathology, Vol. 148, No. 2, 01.08.2017, p. 119-127.

Research output: Contribution to journalArticle

Peterson, Lance R. ; Woods, Christopher W. ; Davis, Thomas ; Wang, Zi Xuam ; Young, Stephen A. ; Osiecki, John C. ; Lewinski, Michael A. ; Liesenfeld, Oliver. / Performance of the cobas MRSA/SA Test for Simultaneous Detection of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus from Nasal Swabs. In: American Journal of Clinical Pathology. 2017 ; Vol. 148, No. 2. pp. 119-127.
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abstract = "Objectives Health care-associated methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus (SA) infections are continuing problems. Rapidly determining the MRSA colonization status of a patient facilitates practice to reduce spread of MRSA clinical disease. Sensitive detection of all SA prior to surgery, followed by decolonization, can significantly reduce postoperative infection from this pathogen. Our goal was to validate a new automated assay for this testing. Methods We compared performance of the cobas MRSA/SA Test on the cobas 4800 System to direct and enriched chromogenic culture using nasal swabs collected from patients at six United States sites. Results Compared to direct and enriched culture, the sensitivity for MRSA and SA was 93.1{\%} and 93.9{\%}, and the specificity was 97.5{\%} and 94.2{\%}, respectively. After discrepancy analysis, the sensitivity for MRSA and SA was 97.1{\%} and 98.6{\%}, and the specificity was 98.3{\%} and 95.5{\%}, respectively. Compared to direct culture, sensitivity for detecting any SA was 99.6{\%}. Conclusions The cobas MRSA/SA Test is an effective tool to simultaneously perform surveillance testing for nasal colonization of both MRSA and MSSA.",
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