Periodate-triggered cross-linking reveals Sug2/Rpt4 as the molecular target of a peptoid inhibitor of the 19S proteasome regulatory particle

Hyun-Suk Lim, Di Cai, Chase T. Archer, Thomas Kodadek

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

This study describes the identification of the protein target of the first chemical inhibitor (RIP-1) of the 19S regulatory particle (RP) of the 26S proteasome. Periodate-triggered chemical cross-linking of DOPA-conjugated RIP-1 and the 26S proteasome identified Sug2/Rpt4, one of the six ATPases in the 19S RP as the molecular target of RIP-1. The specificity of RIP-1 for Sug2/Rpt4 was demonstrated by examining cross-linking reactions with each ATPase of the 19S RP. RIP-1 should provide a useful biological tool to probe the various biological roles of Sug2/Rpt4.

Original languageEnglish (US)
Pages (from-to)12936-12937
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number43
DOIs
StatePublished - Oct 31 2007
Externally publishedYes

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Peptoids
Proteasome Inhibitors
Proteasome Endopeptidase Complex
Adenosine Triphosphatases
Cross Reactions
Proteins
metaperiodate

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Periodate-triggered cross-linking reveals Sug2/Rpt4 as the molecular target of a peptoid inhibitor of the 19S proteasome regulatory particle. / Lim, Hyun-Suk; Cai, Di; Archer, Chase T.; Kodadek, Thomas.

In: Journal of the American Chemical Society, Vol. 129, No. 43, 31.10.2007, p. 12936-12937.

Research output: Contribution to journalArticle

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