Periodate-triggered cross-linking reveals Sug2/Rpt4 as the molecular target of a peptoid inhibitor of the 19S proteasome regulatory particle

Hyun Suk Lim, Di Cai, Chase T. Archer, Thomas Kodadek

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

This study describes the identification of the protein target of the first chemical inhibitor (RIP-1) of the 19S regulatory particle (RP) of the 26S proteasome. Periodate-triggered chemical cross-linking of DOPA-conjugated RIP-1 and the 26S proteasome identified Sug2/Rpt4, one of the six ATPases in the 19S RP as the molecular target of RIP-1. The specificity of RIP-1 for Sug2/Rpt4 was demonstrated by examining cross-linking reactions with each ATPase of the 19S RP. RIP-1 should provide a useful biological tool to probe the various biological roles of Sug2/Rpt4.

Original languageEnglish (US)
Pages (from-to)12936-12937
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number43
DOIs
StatePublished - Oct 31 2007
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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