Periostin (an osteoblast-specific factor) is expressed within the embryonic mouse heart during valve formation

Agnieszka Kruzynska-Frejtag, Michal Machnicki, Rhonda Rogers, Roger R. Markwald, Simon Conway

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

Periostin was originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFβ1) and bone morphogenetic protein (BMP)-2. Likewise the endocardial cushions that form within embryonic heart tube (embryonic day (E)10-13) are formed by the recruitment, attachment and spreading of endocardial cells into the overlying extracellular matrix, in response to secreted growth factors of the TGFβ and BMP families. In order to determine whether periostin is similarly involved in heart morphogenesis, in situ hybridization and reverse transcription-polymerase chain reaction were used to detect periostin mRNA expression in the developing mouse heart. We show for the first time that periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Original languageEnglish (US)
Pages (from-to)183-188
Number of pages6
JournalMechanisms of Development
Volume103
Issue number1-2
DOIs
StatePublished - May 2001
Externally publishedYes

Fingerprint

Heart Valves
Osteoblasts
Endocardial Cushions
Fetal Heart
Bone Morphogenetic Protein 2
Messenger RNA
Bone Morphogenetic Proteins
Cell Adhesion Molecules
Morphogenesis
Transforming Growth Factor beta
Reverse Transcription
In Situ Hybridization
Extracellular Matrix
Intercellular Signaling Peptides and Proteins
Polymerase Chain Reaction

Keywords

  • Bone morphogenetic protein 2
  • Cell adhesion
  • Endocardial cushion
  • Epithelial-mesenchymal transformation
  • Heart
  • Mouse
  • Periostin
  • Transforming growth factor β

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Periostin (an osteoblast-specific factor) is expressed within the embryonic mouse heart during valve formation. / Kruzynska-Frejtag, Agnieszka; Machnicki, Michal; Rogers, Rhonda; Markwald, Roger R.; Conway, Simon.

In: Mechanisms of Development, Vol. 103, No. 1-2, 05.2001, p. 183-188.

Research output: Contribution to journalArticle

Kruzynska-Frejtag, Agnieszka ; Machnicki, Michal ; Rogers, Rhonda ; Markwald, Roger R. ; Conway, Simon. / Periostin (an osteoblast-specific factor) is expressed within the embryonic mouse heart during valve formation. In: Mechanisms of Development. 2001 ; Vol. 103, No. 1-2. pp. 183-188.
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