Periostin contributes to the pathogenesis of atopic dermatitis by inducing TSLP production from keratinocytes

Hiroshi Shiraishi, Miho Masuoka, Shoichiro Ohta, Shoichi Suzuki, Kazuhiko Arima, Kazuto Taniguchi, Shigehisa Aoki, Shuji Toda, Tomohiro Yoshimoto, Naoki Inagaki, Simon Conway, Yutaka Narisawa, Kenji Izuhara

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease where Th2-type immune responses are dominant. Keratinocytes persistently secrete proinflammatory cytokines and chemokines, amplifying Th2-type responses in AD. We have recently reported that periostin, an extracellular matrix protein induced by Th2 cytokines, plays a critical role in AD. In the present study, we have further investigated the characteristics of our allergen-induced AD model mice and the role of periostin in the pathogenesis of AD. Methods: The ears of C57BL/6 mice, BALB/c mice, and Rag-2-/- γc-/- mice (BALB/c background) were epicutaneously sensitized repeatedly with HDM. Mice were analyzed after the final sensitization. To examine the direct role of periostin, we reconstituted skin in vitro by coculture of keratinocytes with wild-type or periostin-deficient fibroblasts. Results: Epicutaneous sensitization with HDM induced AD-like phenotypes and accumulation of periostin in dermis in C57BL/6 mice but not in Rag-2-/- γc-/- mice. In vitro organotypic coculture systems revealed that periostin promoted survival and proliferation of keratinocytes and directly induced production of thymic stromal lymphopoietin (TSLP). Conclusions: Our results suggest that periostin exacerbates the pathogenesis of AD through TSLP production from keratinocytes.

Original languageEnglish
Pages (from-to)563-572
Number of pages10
JournalAllergology International
Volume61
Issue number4
DOIs
StatePublished - 2012

Fingerprint

Atopic Dermatitis
Keratinocytes
Coculture Techniques
Inbred C57BL Mouse
Cytokines
Extracellular Matrix Proteins
Dermis
thymic stromal lymphopoietin
Chemokines
Skin Diseases
Allergens
Ear
Fibroblasts
Phenotype
Skin

Keywords

  • Atopic dermatitis
  • House dust mite
  • Periostin
  • Th2 cells
  • TSLP

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Shiraishi, H., Masuoka, M., Ohta, S., Suzuki, S., Arima, K., Taniguchi, K., ... Izuhara, K. (2012). Periostin contributes to the pathogenesis of atopic dermatitis by inducing TSLP production from keratinocytes. Allergology International, 61(4), 563-572. https://doi.org/10.2332/allergolint.10-OA-0297

Periostin contributes to the pathogenesis of atopic dermatitis by inducing TSLP production from keratinocytes. / Shiraishi, Hiroshi; Masuoka, Miho; Ohta, Shoichiro; Suzuki, Shoichi; Arima, Kazuhiko; Taniguchi, Kazuto; Aoki, Shigehisa; Toda, Shuji; Yoshimoto, Tomohiro; Inagaki, Naoki; Conway, Simon; Narisawa, Yutaka; Izuhara, Kenji.

In: Allergology International, Vol. 61, No. 4, 2012, p. 563-572.

Research output: Contribution to journalArticle

Shiraishi, H, Masuoka, M, Ohta, S, Suzuki, S, Arima, K, Taniguchi, K, Aoki, S, Toda, S, Yoshimoto, T, Inagaki, N, Conway, S, Narisawa, Y & Izuhara, K 2012, 'Periostin contributes to the pathogenesis of atopic dermatitis by inducing TSLP production from keratinocytes', Allergology International, vol. 61, no. 4, pp. 563-572. https://doi.org/10.2332/allergolint.10-OA-0297
Shiraishi, Hiroshi ; Masuoka, Miho ; Ohta, Shoichiro ; Suzuki, Shoichi ; Arima, Kazuhiko ; Taniguchi, Kazuto ; Aoki, Shigehisa ; Toda, Shuji ; Yoshimoto, Tomohiro ; Inagaki, Naoki ; Conway, Simon ; Narisawa, Yutaka ; Izuhara, Kenji. / Periostin contributes to the pathogenesis of atopic dermatitis by inducing TSLP production from keratinocytes. In: Allergology International. 2012 ; Vol. 61, No. 4. pp. 563-572.
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AU - Masuoka, Miho

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AU - Suzuki, Shoichi

AU - Arima, Kazuhiko

AU - Taniguchi, Kazuto

AU - Aoki, Shigehisa

AU - Toda, Shuji

AU - Yoshimoto, Tomohiro

AU - Inagaki, Naoki

AU - Conway, Simon

AU - Narisawa, Yutaka

AU - Izuhara, Kenji

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