Periostin modulates myofibroblast differentiation during full-thickness cutaneous wound repair

Christopher G. Elliott, Jian Wang, Xiaolei Guo, Shi wen Xu, Mark Eastwood, Jianjun Guan, Andrew Leask, Simon J. Conway, Douglas W. Hamilton

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

The matricellular protein periostin is expressed in the skin. Although periostin has been hypothesized to contribute to dermal homeostasis and repair, this has not been directly tested. To assess the contribution of periostin to dermal healing, 6 mm full-thickness excisional wounds were created in the skin of periostin-knockout and wild-type, sex-matched control mice. In wild-type mice, periostin was potently induced 5-7 days after wounding. In the absence of periostin, day 7 wounds showed a significant reduction in myofibroblasts, as visualized by expression of α-smooth muscle actin (α-SMA) within the granulation tissue. Delivery of recombinant human periostin by electrospun collagen scaffolds restored α-SMA expression. Isolated wild-type and knockout dermal fibroblasts did not differ in in vitro assays of adhesion or migration; however, in 3D culture, periostin-knockout fibroblasts showed a significantly reduced ability to contract a collagen matrix, and adopted a dendritic phenotype. Recombinant periostin restored the defects in cell morphology and matrix contraction displayed by periostin-deficient fibroblasts in a manner that was sensitive to a neutralizing anti-β1-integrin and to the FAK and Src inhibitor PP2. We propose that periostin promotes wound contraction by facilitating myofibroblast differentiation and contraction.

Original languageEnglish (US)
Pages (from-to)121-132
Number of pages12
JournalJournal of cell science
Volume125
Issue number1
DOIs
StatePublished - Jan 1 2012

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Keywords

  • α-smooth muscle actin
  • Contraction
  • Matricellular protein
  • Myofibroblast
  • Periostin
  • Wound healing

ASJC Scopus subject areas

  • Cell Biology

Cite this

Elliott, C. G., Wang, J., Guo, X., Xu, S. W., Eastwood, M., Guan, J., Leask, A., Conway, S. J., & Hamilton, D. W. (2012). Periostin modulates myofibroblast differentiation during full-thickness cutaneous wound repair. Journal of cell science, 125(1), 121-132. https://doi.org/10.1242/jcs.087841