Peripheral administration of ethanol results in a correlated increase in dopamine and serotonin within the posterior ventral tegmental area

Gerald A. Deehan, Christopher P. Knight, R. Aaron Waeiss, Eric Engleman, Jamie E. Toalston, William J. McBride, Sheketha R. Hauser, Zachary Rodd

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aims: Two critical neurotransmitter systems regulating ethanol (EtOH) reward are serotonin (5-HT) and dopamine (DA). Within the posterior ventral tegmental area (pVTA), 5-HT receptors have been shown to regulate DA neuronal activity. Increased pVTA neuronal activity has been linked to drug reinforcement. The current experiment sought to determine the effect of EtOH on 5-HT and DA levels within the pVTA.Methods: Wistar rats were implanted with cannula aimed at the pVTA. Neurochemical levels were determined using standard microdialysis procedures with concentric probes. Rats were randomly assigned to one of the five groups (n = 41; 7-9 per group) that were treated with 0-3.0 g/kg EtOH (intraperitoneally).Results: Ethanol produced increased extracellular DA levels in the pVTA that resembled an inverted U-shape dose-response curve with peak levels (~200% of baseline) at the 2.25 g/kg dose. The increase in DA levels was observed for an extended period of time (~100 minutes). The effects of EtOH on extracellular 5-HT levels in the pVTA also resembled an inverted U-shape dose-response curve. However, increased 5-HT levels were only observed during the initial post-injection sample. The increases in extracellular DA and 5-HT levels were significantly correlated.Conclusion: The data indicate intraperitoneal EtOH administration stimulated the release of both 5-HT and DA within the pVTA, the levels of which were significantly correlated. Overall, the current findings suggest that the ability of EtOH to stimulate DA activity within the mesolimbic system may be modulated by increases in 5-HT release within the pVTA. Short summary: Two critical neurotransmitter systems regulating ethanol reward are serotonin and dopamine. The current experiment determined that intraperitoneal ethanol administration increased serotonin and dopamine levels within the pVTA (levels were significantly correlated). The current findings suggest the ability of EtOH to stimulate serotonin and dopamine activity within the mesolimbic system.

Original languageEnglish (US)
Article numberagw037
Pages (from-to)535-540
Number of pages6
JournalAlcohol and Alcoholism
Volume51
Issue number5
DOIs
StatePublished - Sep 1 2016

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Ventral Tegmental Area
Dopamine
Serotonin
Ethanol
Aptitude
Reward
Neurotransmitter Agents
Rats
Serotonin Receptors
Microdialysis
Wistar Rats
Reinforcement
Experiments

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Medicine(all)
  • Toxicology
  • Psychiatry and Mental health

Cite this

Peripheral administration of ethanol results in a correlated increase in dopamine and serotonin within the posterior ventral tegmental area. / Deehan, Gerald A.; Knight, Christopher P.; Waeiss, R. Aaron; Engleman, Eric; Toalston, Jamie E.; McBride, William J.; Hauser, Sheketha R.; Rodd, Zachary.

In: Alcohol and Alcoholism, Vol. 51, No. 5, agw037, 01.09.2016, p. 535-540.

Research output: Contribution to journalArticle

Deehan, Gerald A. ; Knight, Christopher P. ; Waeiss, R. Aaron ; Engleman, Eric ; Toalston, Jamie E. ; McBride, William J. ; Hauser, Sheketha R. ; Rodd, Zachary. / Peripheral administration of ethanol results in a correlated increase in dopamine and serotonin within the posterior ventral tegmental area. In: Alcohol and Alcoholism. 2016 ; Vol. 51, No. 5. pp. 535-540.
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T1 - Peripheral administration of ethanol results in a correlated increase in dopamine and serotonin within the posterior ventral tegmental area

AU - Deehan, Gerald A.

AU - Knight, Christopher P.

AU - Waeiss, R. Aaron

AU - Engleman, Eric

AU - Toalston, Jamie E.

AU - McBride, William J.

AU - Hauser, Sheketha R.

AU - Rodd, Zachary

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AB - Aims: Two critical neurotransmitter systems regulating ethanol (EtOH) reward are serotonin (5-HT) and dopamine (DA). Within the posterior ventral tegmental area (pVTA), 5-HT receptors have been shown to regulate DA neuronal activity. Increased pVTA neuronal activity has been linked to drug reinforcement. The current experiment sought to determine the effect of EtOH on 5-HT and DA levels within the pVTA.Methods: Wistar rats were implanted with cannula aimed at the pVTA. Neurochemical levels were determined using standard microdialysis procedures with concentric probes. Rats were randomly assigned to one of the five groups (n = 41; 7-9 per group) that were treated with 0-3.0 g/kg EtOH (intraperitoneally).Results: Ethanol produced increased extracellular DA levels in the pVTA that resembled an inverted U-shape dose-response curve with peak levels (~200% of baseline) at the 2.25 g/kg dose. The increase in DA levels was observed for an extended period of time (~100 minutes). The effects of EtOH on extracellular 5-HT levels in the pVTA also resembled an inverted U-shape dose-response curve. However, increased 5-HT levels were only observed during the initial post-injection sample. The increases in extracellular DA and 5-HT levels were significantly correlated.Conclusion: The data indicate intraperitoneal EtOH administration stimulated the release of both 5-HT and DA within the pVTA, the levels of which were significantly correlated. Overall, the current findings suggest that the ability of EtOH to stimulate DA activity within the mesolimbic system may be modulated by increases in 5-HT release within the pVTA. Short summary: Two critical neurotransmitter systems regulating ethanol reward are serotonin and dopamine. The current experiment determined that intraperitoneal ethanol administration increased serotonin and dopamine levels within the pVTA (levels were significantly correlated). The current findings suggest the ability of EtOH to stimulate serotonin and dopamine activity within the mesolimbic system.

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