Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses

Ana Leda F. Longhini, Tatiana E. Salazar, Cristiano Vieira, Thao Trinh, Yaqian Duan, Louise M. Pay, Sergio Li Calzi, Megan Losh, Nancy A. Johnston, Huisheng Xie, Minsu Kim, Robert J. Hunt, Mervin Yoder, Domenico Santoro, Taralyn M. McCarrel, Maria B. Grant

Research output: Contribution to journalArticle

Abstract

Previously, we showed that mesenchymal stem cells (MSC) can be mobilized into peripheral blood using electroacupuncture (EA) at acupoints, LI-4, LI-11, GV-14, and GV-20. The purpose of this study was to determine whether EA-mobilized MSC could be harvested and expanded in vitro to be used as an autologous cell therapy in horses. Peripheral blood mononuclear cells (PBMC) isolated from young and aged lame horses (n = 29) showed a marked enrichment for MSCs. MSC were expanded in vitro (n = 25) and administered intravenously at a dose of 50 x 10 6 (n = 24). Treatment resulted in significant improvement in lameness as assessed by the American Association of Equine Practitioners (AAEP) lameness scale (n = 23). MSCs exhibited immunomodulatory function by inhibition of lymphocyte proliferation and induction of IL-10. Intradermal testing showed no immediate or delayed immune reactions to MSC (1 x 10 6 to 1 x 10 4 ). In this study, we demonstrated an efficient, safe and reproducible method to mobilize and expand, in vitro, MSCs in sufficiently high concentrations for therapeutic administration. We confirm the immunomodulatory function of these cells in vitro. This non-pharmacological and non-surgical strategy for stem cell harvest has a broad range of biomedical applications and represents an improved clinically translatable and economical cell source for humans.

Original languageEnglish (US)
Article numbere0212642
JournalPLoS ONE
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2019

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Therapeutic Uses
Stem cells
Mesenchymal Stromal Cells
Horses
stem cells
Blood
Electroacupuncture
horses
therapeutics
blood
lameness
Acupuncture Points
Antigen-antibody reactions
Cell- and Tissue-Based Therapy
Interleukin-10
Lymphocytes
cells
Blood Cells
lymphocyte proliferation
mononuclear leukocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Longhini, A. L. F., Salazar, T. E., Vieira, C., Trinh, T., Duan, Y., Pay, L. M., ... Grant, M. B. (2019). Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. PLoS ONE, 14(3), [e0212642]. https://doi.org/10.1371/journal.pone.0212642

Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. / Longhini, Ana Leda F.; Salazar, Tatiana E.; Vieira, Cristiano; Trinh, Thao; Duan, Yaqian; Pay, Louise M.; Calzi, Sergio Li; Losh, Megan; Johnston, Nancy A.; Xie, Huisheng; Kim, Minsu; Hunt, Robert J.; Yoder, Mervin; Santoro, Domenico; McCarrel, Taralyn M.; Grant, Maria B.

In: PLoS ONE, Vol. 14, No. 3, e0212642, 01.03.2019.

Research output: Contribution to journalArticle

Longhini, ALF, Salazar, TE, Vieira, C, Trinh, T, Duan, Y, Pay, LM, Calzi, SL, Losh, M, Johnston, NA, Xie, H, Kim, M, Hunt, RJ, Yoder, M, Santoro, D, McCarrel, TM & Grant, MB 2019, 'Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses', PLoS ONE, vol. 14, no. 3, e0212642. https://doi.org/10.1371/journal.pone.0212642
Longhini, Ana Leda F. ; Salazar, Tatiana E. ; Vieira, Cristiano ; Trinh, Thao ; Duan, Yaqian ; Pay, Louise M. ; Calzi, Sergio Li ; Losh, Megan ; Johnston, Nancy A. ; Xie, Huisheng ; Kim, Minsu ; Hunt, Robert J. ; Yoder, Mervin ; Santoro, Domenico ; McCarrel, Taralyn M. ; Grant, Maria B. / Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. In: PLoS ONE. 2019 ; Vol. 14, No. 3.
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AU - Trinh, Thao

AU - Duan, Yaqian

AU - Pay, Louise M.

AU - Calzi, Sergio Li

AU - Losh, Megan

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AU - Xie, Huisheng

AU - Kim, Minsu

AU - Hunt, Robert J.

AU - Yoder, Mervin

AU - Santoro, Domenico

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