Perivascular T-cell infiltration leads to sustained pulmonary artery remodeling after endothelial cell damage

Michael J. Cuttica, Thomas Langenickel, Audrey Noguchi, Roberto Machado, Mark T. Gladwin, Manfred Boehm

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Pulmonary hypertension is a vascular proliferative disease characterized by pulmonary artery remodeling because of dysregulated endothelial and smooth muscle cell proliferation. Although the role of inflammation in the development of the disease is not well-defined, plexogenic lesions in human disease are characterized by perivascular inflammation composed, in part, of T cells. We explored the role of T-cell infiltration on pulmonary vascular remodeling after endothelial cell damage. We induced endothelial cell damage using monocrotaline and isolated the role of T cells by using Rag1tm1Mom mice and performing adoptive T-cell transfer. We found that monocrotaline causes pulmonary vascular endothelial cell injury followed by a perivascular inflammatory response. The infiltration of inflammatory cells primarily involves CD4+ T cells and leads to the progressive muscularization of small (<30 μm) arterioles. Pulmonary vascular proliferative changes were accompanied by progressive and persistent elevations in right ventricular pressure and right ventricular hypertrophy. Supporting the central role of CD4+ T cells in the inflammatory response, Rag1tm1Mom (Rag1-/-) mice, which are devoid of T and B cells, were protected from the development of vascular injury when exposed to monocrotaline. The introduction of T cells from control mice into Rag1-/- mice reproduced the vascular injury phenotype. These data indicate that after endothelial cell damage, CD4+ T-cell infiltration participates in pulmonary vascular remodeling. This finding suggests that a CD41 T-cell immune responsemaycontribute to the pathogenesis of inflammatory vascular lesions seen in some forms of pulmonary hypertension.

Original languageEnglish (US)
Pages (from-to)62-71
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume45
Issue number1
DOIs
StatePublished - Jul 1 2011
Externally publishedYes

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T-cells
Endothelial cells
Infiltration
Pulmonary Artery
Endothelial Cells
T-Lymphocytes
Monocrotaline
Lung
Vascular System Injuries
Pulmonary Hypertension
Blood Vessels
Inflammation
Right Ventricular Hypertrophy
Cell proliferation
Adoptive Transfer
Arterioles
Ventricular Pressure
Vascular Diseases
Muscle
Smooth Muscle Myocytes

Keywords

  • CD4+ T cells
  • Inflammation
  • Pulmonary vascular remodeling
  • Rag1 mice

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Perivascular T-cell infiltration leads to sustained pulmonary artery remodeling after endothelial cell damage. / Cuttica, Michael J.; Langenickel, Thomas; Noguchi, Audrey; Machado, Roberto; Gladwin, Mark T.; Boehm, Manfred.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 45, No. 1, 01.07.2011, p. 62-71.

Research output: Contribution to journalArticle

Cuttica, Michael J. ; Langenickel, Thomas ; Noguchi, Audrey ; Machado, Roberto ; Gladwin, Mark T. ; Boehm, Manfred. / Perivascular T-cell infiltration leads to sustained pulmonary artery remodeling after endothelial cell damage. In: American Journal of Respiratory Cell and Molecular Biology. 2011 ; Vol. 45, No. 1. pp. 62-71.
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