PET imaging and optical imaging with D-luciferin [11C]methyl ester and D-luciferin [11C]methyl ether of luciferase gene expression in tumor xenografts of living mice

Ji Quan Wang, Karen Pollok, Shanbao Cai, Keith M. Stantz, Gary Hutchins, Qi-Huang Zheng

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

New carbon-11 labeled d-luciferin analogs d-luciferin [11C] methyl ester ([11C]LMEster, [11C]1) and d-luciferin [ 11C]methyl ether ([11C]LMEther, [11C]2) were synthesized in 25-55% radiochemical yield. PET studies with [ 11C]LMEster and [11C]LMEther demonstrate a lower retention of the C-11 label at 45 min post-injection in luciferase expression tumor. Optical imaging with unlabeled substrate d-luciferin and radiotracers [ 11C]LMEster and [11C]LMEther gave tumor luciferase images within a few minutes of photon counting.

Original languageEnglish
Pages (from-to)331-337
Number of pages7
JournalBioorganic and Medicinal Chemistry Letters
Volume16
Issue number2
DOIs
StatePublished - Jan 15 2006

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Methyl Ethers
Optical Imaging
Luciferases
Heterografts
Gene expression
Tumors
Esters
Gene Expression
Imaging techniques
Photons
Labels
Neoplasms
Carbon
Injections
Substrates
D-luciferin

Keywords

  • D-Luciferin [C]methyl ester ([C]LMEster, [ C]1)
  • d-Luciferin [C]methyl ether ([ C]LMEther, [C]2)
  • Gene expression
  • Luciferase
  • Positron emission tomography (PET) imaging

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

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title = "PET imaging and optical imaging with D-luciferin [11C]methyl ester and D-luciferin [11C]methyl ether of luciferase gene expression in tumor xenografts of living mice",
abstract = "New carbon-11 labeled d-luciferin analogs d-luciferin [11C] methyl ester ([11C]LMEster, [11C]1) and d-luciferin [ 11C]methyl ether ([11C]LMEther, [11C]2) were synthesized in 25-55{\%} radiochemical yield. PET studies with [ 11C]LMEster and [11C]LMEther demonstrate a lower retention of the C-11 label at 45 min post-injection in luciferase expression tumor. Optical imaging with unlabeled substrate d-luciferin and radiotracers [ 11C]LMEster and [11C]LMEther gave tumor luciferase images within a few minutes of photon counting.",
keywords = "D-Luciferin [C]methyl ester ([C]LMEster, [ C]1), d-Luciferin [C]methyl ether ([ C]LMEther, [C]2), Gene expression, Luciferase, Positron emission tomography (PET) imaging",
author = "Wang, {Ji Quan} and Karen Pollok and Shanbao Cai and Stantz, {Keith M.} and Gary Hutchins and Qi-Huang Zheng",
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T1 - PET imaging and optical imaging with D-luciferin [11C]methyl ester and D-luciferin [11C]methyl ether of luciferase gene expression in tumor xenografts of living mice

AU - Wang, Ji Quan

AU - Pollok, Karen

AU - Cai, Shanbao

AU - Stantz, Keith M.

AU - Hutchins, Gary

AU - Zheng, Qi-Huang

PY - 2006/1/15

Y1 - 2006/1/15

N2 - New carbon-11 labeled d-luciferin analogs d-luciferin [11C] methyl ester ([11C]LMEster, [11C]1) and d-luciferin [ 11C]methyl ether ([11C]LMEther, [11C]2) were synthesized in 25-55% radiochemical yield. PET studies with [ 11C]LMEster and [11C]LMEther demonstrate a lower retention of the C-11 label at 45 min post-injection in luciferase expression tumor. Optical imaging with unlabeled substrate d-luciferin and radiotracers [ 11C]LMEster and [11C]LMEther gave tumor luciferase images within a few minutes of photon counting.

AB - New carbon-11 labeled d-luciferin analogs d-luciferin [11C] methyl ester ([11C]LMEster, [11C]1) and d-luciferin [ 11C]methyl ether ([11C]LMEther, [11C]2) were synthesized in 25-55% radiochemical yield. PET studies with [ 11C]LMEster and [11C]LMEther demonstrate a lower retention of the C-11 label at 45 min post-injection in luciferase expression tumor. Optical imaging with unlabeled substrate d-luciferin and radiotracers [ 11C]LMEster and [11C]LMEther gave tumor luciferase images within a few minutes of photon counting.

KW - D-Luciferin [C]methyl ester ([C]LMEster, [ C]1)

KW - d-Luciferin [C]methyl ether ([ C]LMEther, [C]2)

KW - Gene expression

KW - Luciferase

KW - Positron emission tomography (PET) imaging

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U2 - 10.1016/j.bmcl.2005.09.082

DO - 10.1016/j.bmcl.2005.09.082

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JO - Bioorganic and Medicinal Chemistry Letters

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SN - 0960-894X

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