PGE2 is essential for gap junction-mediated Intercellular communication between osteocyte-like MLO-Y4 cells in response to mechanical strain

Benxu Cheng, Yoichi Kato, Shujie Zhao, Jian Luo, Eugene Sprague, Lynda Bonewald, Jean X. Jiang

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

We have observed, in our previous studies, that fluid flow increases gap junction-mediated intercellular coupling and the expression of a gap junction protein, connexin 43, in osteocyte-like MLO-Y4 cells. Interestingly, this stimulation is further enhanced during the poststress period, indicating that a released factor(s) is likely to be involved. Here, we report that the conditioned medium obtained from the fluid flow-treated MLO-Y4 cells increased the number of functional gap junctions and connexin 43 protein. These changes are similar to those observed in MLO-Y4 cells directly exposed to fluid flow. Fluid flow was found to induce PGE2 release and increase cyclooxygenase 2 expression. Treatment of the cells with PGE2 had the same effect as fluid flow, suggesting that PGE2 could be responsible for these autocrine effects. When PGE2 was depleted from the fluid flow-conditioned medium, the stimulatory effect on gap junctions was partially, but significantly, decreased. Addition of the cyclooxygenase inhibitor, indomethacin, partially blocked the stimulatory effects of mechanical strain on gap junctions. Taken together, these studies suggest that the stimulatory effect of fluid flow on gap junctions is mediated, in part, by the release of PGE2. Hence, PGE2 is an essential mediator between mechanical strain and gap junctions in osteocyte-like cells.

Original languageEnglish (US)
Pages (from-to)3464-3473
Number of pages10
JournalEndocrinology
Volume142
Issue number8
DOIs
StatePublished - 2001
Externally publishedYes

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Osteocytes
Gap Junctions
Dinoprostone
Connexin 43
Conditioned Culture Medium
Connexins
Cyclooxygenase Inhibitors
Cyclooxygenase 2
Indomethacin
Cell Count
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

PGE2 is essential for gap junction-mediated Intercellular communication between osteocyte-like MLO-Y4 cells in response to mechanical strain. / Cheng, Benxu; Kato, Yoichi; Zhao, Shujie; Luo, Jian; Sprague, Eugene; Bonewald, Lynda; Jiang, Jean X.

In: Endocrinology, Vol. 142, No. 8, 2001, p. 3464-3473.

Research output: Contribution to journalArticle

Cheng, Benxu ; Kato, Yoichi ; Zhao, Shujie ; Luo, Jian ; Sprague, Eugene ; Bonewald, Lynda ; Jiang, Jean X. / PGE2 is essential for gap junction-mediated Intercellular communication between osteocyte-like MLO-Y4 cells in response to mechanical strain. In: Endocrinology. 2001 ; Vol. 142, No. 8. pp. 3464-3473.
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