Phagocytic activation of human neutrophils by the detergent component of fluosol

David Ingram, Mervyn B. Forman, John J. Murray

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Fluosol (Alpha Therapeutic Corporation, Los Angeles, CA) an emulsion of perfluorocarbons with a high oxygen-carrying capacity, was approved as an adjunct to alleviate myocardial ischemia during coronary angioplasty. This drug also significantly enhances myocardial salvage presumably related to an action on the neutrophil. The mechanism by which fluosol and its individual components, including the detergent Pluronic F-68, affected neutrophil function was examined. During the incubation of neutrophils with fluosol, a rapid stimulation of superoxide anion production and degranulation which progressively increased over a 30-minute period was detected. Neutrophils incubated with only Pluronic F-68 produced similar amounts of superoxide anion. Cytochalasin B, an inhibitor of phagocytosis, significantly inhibited this superoxide union generation. As shown previously, neutrophils incubated with fluosol for 30 minutes and then subsequently stimulated manifested a reduction in lysozyme release as compared with untreated cells. Results of an electron microscopic examination confirmed the cellular uptake of the fluosol within phagocytic vacuoles. Neutrophil viability determined by trypan blue was unaffected after fluosol treatment. These observations show that the fluosol emulsion, primarily through micelles formed by the detergent Pluronic F-68, activates human neutrophils by serving as a phagocytic stimulus, which produces a cell refractory to subsequent stimulation.

Original languageEnglish (US)
Pages (from-to)1081-1087
Number of pages7
JournalAmerican Journal of Pathology
Volume140
Issue number5
StatePublished - May 1992
Externally publishedYes

Fingerprint

Neutrophil Activation
Detergents
Neutrophils
Poloxamer
Superoxides
Emulsions
Fluorocarbons
Cytochalasin B
Trypan Blue
Los Angeles
Conservation of Natural Resources
Micelles
Muramidase
Vacuoles
fluosol
Angioplasty
Phagocytosis
Myocardial Ischemia
Electrons
Oxygen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Phagocytic activation of human neutrophils by the detergent component of fluosol. / Ingram, David; Forman, Mervyn B.; Murray, John J.

In: American Journal of Pathology, Vol. 140, No. 5, 05.1992, p. 1081-1087.

Research output: Contribution to journalArticle

Ingram, David ; Forman, Mervyn B. ; Murray, John J. / Phagocytic activation of human neutrophils by the detergent component of fluosol. In: American Journal of Pathology. 1992 ; Vol. 140, No. 5. pp. 1081-1087.
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