Phagocytosis of different matrix components by different cell types at bone-forming sites in cultured mouse calvariae

Tsuneo Takahashi, Seigyo So, Duenjeng Wang, Kazuto Takahashi, Noriyoshi Kurihara, Masayoshi Kumegawa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Sites of bone formation on fragments of parietal bone of fetal-mice cultured for 10 days were examined by electron microscopy after addition of either ruthenium red or ferrocyanide to the postfixation fluid. Osteoclasts, osteoblast-like cells, and macrophages were the principal active cells at these formation sites. The mononuclear cells (osteoblast-like cells and macrophages) in the osteoid tissue showed evidence of having incorporated elements of calcified tissue. Osteoblast-like cells had phagocytized collagen fibrils and calcified bone matrix. This occurred more frequently in the calcifying area. Mononuclear macrophages showed not only phagocytosis and digestion of cellular debris and bone spicules in the osteoid, but also active incorporation of calcified bone matrix that had been detached from its surroundings by its pseudopod-like projections from long cytoplasmic processes. Collagen fibrils were seldom observed within the macrophages. These observations suggest that in our culture system osteoblast-like cells and macrophages at bone formation sites have a phagocytic role in bone remodeling.

Original languageEnglish (US)
Pages (from-to)9-17
Number of pages9
JournalCell and Tissue Research
Volume245
Issue number1
DOIs
StatePublished - Jul 1986
Externally publishedYes

Fingerprint

Cellular Structures
Phagocytosis
Skull
Bone
Macrophages
Osteoblasts
Bone and Bones
Bone Matrix
Osteogenesis
Collagen
Parietal Bone
Ruthenium Red
Pseudopodia
Tissue
Bone Remodeling
Osteoclasts
Digestion
Cell culture
Debris
Electron Microscopy

Keywords

  • Bone formation
  • Calvaria
  • Macrophage
  • Mouse
  • Osteoblast-like cell
  • Osteoid

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Phagocytosis of different matrix components by different cell types at bone-forming sites in cultured mouse calvariae. / Takahashi, Tsuneo; So, Seigyo; Wang, Duenjeng; Takahashi, Kazuto; Kurihara, Noriyoshi; Kumegawa, Masayoshi.

In: Cell and Tissue Research, Vol. 245, No. 1, 07.1986, p. 9-17.

Research output: Contribution to journalArticle

Takahashi, Tsuneo ; So, Seigyo ; Wang, Duenjeng ; Takahashi, Kazuto ; Kurihara, Noriyoshi ; Kumegawa, Masayoshi. / Phagocytosis of different matrix components by different cell types at bone-forming sites in cultured mouse calvariae. In: Cell and Tissue Research. 1986 ; Vol. 245, No. 1. pp. 9-17.
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