Phagocytosis of different matrix components by different cell types at bone-forming sites in cultured mouse calvariae

Tsuneo Takahashi, Seigyo So, Duenjeng Wang, Kazuto Takahashi, Noriyoshi Kurihara, Masayoshi Kumegawa

Research output: Contribution to journalArticle

21 Scopus citations


Sites of bone formation on fragments of parietal bone of fetal-mice cultured for 10 days were examined by electron microscopy after addition of either ruthenium red or ferrocyanide to the postfixation fluid. Osteoclasts, osteoblast-like cells, and macrophages were the principal active cells at these formation sites. The mononuclear cells (osteoblast-like cells and macrophages) in the osteoid tissue showed evidence of having incorporated elements of calcified tissue. Osteoblast-like cells had phagocytized collagen fibrils and calcified bone matrix. This occurred more frequently in the calcifying area. Mononuclear macrophages showed not only phagocytosis and digestion of cellular debris and bone spicules in the osteoid, but also active incorporation of calcified bone matrix that had been detached from its surroundings by its pseudopod-like projections from long cytoplasmic processes. Collagen fibrils were seldom observed within the macrophages. These observations suggest that in our culture system osteoblast-like cells and macrophages at bone formation sites have a phagocytic role in bone remodeling.

Original languageEnglish (US)
Pages (from-to)9-17
Number of pages9
JournalCell And Tissue Research
Issue number1
StatePublished - Jul 1 1986
Externally publishedYes


  • Bone formation
  • Calvaria
  • Macrophage
  • Mouse
  • Osteoblast-like cell
  • Osteoid

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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