Phase-sensitive optical coherence tomography characterization of pulse-induced trabecular meshwork displacement in ex vivononhuman primate eyes

Peng Li, Roberto Reif, Zhongwei Zhi, Elizabeth Martin, Tueng T. Shen, Murray Johnstone, Ruikang K. Wang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Glaucoma is a blinding disease for which intraocular pressure (IOP) is the only treatable risk factor. The mean IOP is regulated through the aqueous outflow system, which contains the trabecular meshwork (TM). Considerable evidence indicates that trabecular tissue movement regulates the aqueous outflow and becomes abnormal during glaucoma; however, such motion has thus far escaped detection. The purpose of this study is to describe anovel use of a phase-sensitive optical coherence tomography (PhS-OCT) method to assess pulse-dependent TM movement. For this study, we used enucleated monkey eyes, each mounted in an anterior segment holder. A perfusion system was used to control the mean IOP as well as to provide IOP sinusoidal transients (amplitude 3 mmHg, frequency 1 pulse/second) in all experiments. Measurements were carried out at seven graded mean IOPs (5, 8, 10, 20, 30, 40, and 50 mm Hg). We demonstrate that PhS-OCT is sensitive enough to image/visualize TM movement synchronous with the pulse-induced IOP transients, providing quantitative measurements of dynamic parameters such as velocity, displacement, and strain rate that are important for assessing the biome-chanical compliance of the TM. We find that the largest TM displacement is in the area closest to Schlemm's canal (SC) endothelium. While maintaining constant ocular pulse amplitude, an increase of mean IOP results in a decrease of TM displacement and mean size of the SC. These results demonstrate that the PhS-OCT is a useful imaging technique capable of assessing functional properties necessary to maintain IOP in a healthy range, offering a new diagnostic alternative for glaucoma.

Original languageEnglish (US)
Article number076026
JournalJournal of Biomedical Optics
Volume17
Issue number7
DOIs
StatePublished - Jul 1 2012
Externally publishedYes

Fingerprint

intraocular pressure
primates
Optical tomography
tomography
glaucoma
pulses
canals
Canals
endothelium
monkeys
pulse amplitude
holders
Primates
imaging techniques
strain rate
Strain rate
Tissue
Imaging techniques

Keywords

  • Aqueous
  • Glaucoma
  • Optical coherence tomography
  • Pulsatile flow
  • Schlemm's canal
  • Trabecular meshwork

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Phase-sensitive optical coherence tomography characterization of pulse-induced trabecular meshwork displacement in ex vivononhuman primate eyes. / Li, Peng; Reif, Roberto; Zhi, Zhongwei; Martin, Elizabeth; Shen, Tueng T.; Johnstone, Murray; Wang, Ruikang K.

In: Journal of Biomedical Optics, Vol. 17, No. 7, 076026, 01.07.2012.

Research output: Contribution to journalArticle

Li, Peng ; Reif, Roberto ; Zhi, Zhongwei ; Martin, Elizabeth ; Shen, Tueng T. ; Johnstone, Murray ; Wang, Ruikang K. / Phase-sensitive optical coherence tomography characterization of pulse-induced trabecular meshwork displacement in ex vivononhuman primate eyes. In: Journal of Biomedical Optics. 2012 ; Vol. 17, No. 7.
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