Phenotypic evaluation of chronic myeloid leukemia

M. A S Moore, R. Mertelsmann, Louis Pelus

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Marrow culture studies revealed a spectrum of qualitative and quantitative defects in granulocyte-macrophage progenitors (GM-CFC) of patients with chronic myeloid leukemia in chronic phase and blastic crisis. Parallel culture studies and terminal transferase determinations revealed that a significant proportion of patients in blastic crisis possess two coexisting acute phase clones, one lymphoblastic and one myeloblastic. Measurement of response to and production of T cell growth factor showed that the leukemic blast cells from patients with TdT-positive blastic crisis produced the factor, but did not exhibit a proliferative response to exogenous factor. This phenotype was identical to that observed in TdT-positive acute lymphoblastic leukemia. Additional regulatory defects were identified in CML, since leukemic GM-CFC proliferation was resistant to inhibition by concentrations of prostaglandin E, which are markedly inhibitory for normal GM-CFC. The self-renewal or recloning capacity of GM-CFC was also identified as a unique feature of some patients with CML. The addition of retinoic acid to primary cultures of leukemic GM-CFC completely abolished this recloning capacity.

Original languageEnglish (US)
Pages (from-to)217-236
Number of pages20
JournalBlood Cells
Volume7
Issue number2
StatePublished - 1981
Externally publishedYes

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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Leukemia, Myeloid, Chronic Phase
Granulocyte-Macrophage Progenitor Cells
Transferases
Prostaglandins E
Tretinoin
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Interleukin-2
Clone Cells
Bone Marrow
Phenotype

ASJC Scopus subject areas

  • Hematology

Cite this

Moore, M. A. S., Mertelsmann, R., & Pelus, L. (1981). Phenotypic evaluation of chronic myeloid leukemia. Blood Cells, 7(2), 217-236.

Phenotypic evaluation of chronic myeloid leukemia. / Moore, M. A S; Mertelsmann, R.; Pelus, Louis.

In: Blood Cells, Vol. 7, No. 2, 1981, p. 217-236.

Research output: Contribution to journalArticle

Moore, MAS, Mertelsmann, R & Pelus, L 1981, 'Phenotypic evaluation of chronic myeloid leukemia', Blood Cells, vol. 7, no. 2, pp. 217-236.
Moore MAS, Mertelsmann R, Pelus L. Phenotypic evaluation of chronic myeloid leukemia. Blood Cells. 1981;7(2):217-236.
Moore, M. A S ; Mertelsmann, R. ; Pelus, Louis. / Phenotypic evaluation of chronic myeloid leukemia. In: Blood Cells. 1981 ; Vol. 7, No. 2. pp. 217-236.
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