Phenotypic variation of fluoride responses between inbred strains of mice

Dong Yan, Thomas L. Willett, Xiao Mei Gu, Esperanza Martinez Mier, Laura Sardone, Lauren McShane, Marc Grynpas, Eric T. Everett

Research output: Contribution to journalArticle

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Abstract

Excessive systemic exposure to fluoride (F) can lead to disturbances in bone homeostasis and dental enamel development. We have previously shown strain-specific responses to F in the development of dental fluorosis (DF) and in bone formation/mineralization. The current study was undertaken to further investigate F responsive variations in bone metabolism and to determine possible relationships with DF susceptibility. Seven-week-old male mice from FVB/NJ, C57BL/6J, C3H/HeJ, A/J, 129S1/SvImJ, AKR/J, DBA/2J, and BALB/cByJ inbred strains were exposed to NaF (0 or 50 ppm as F -) in drinking water for 60 days. Sera were collected for F, Ca, Mg, PO 4, iPTH, sRANKL, and ALP levels. Bone marrow cells were subjected to ex vivo cell culture for osteoclast potential and CFU colony assays (CFU-fibroblast, CFU-osteoblast, CFU-erythrocyte/granulocyte/macrophage/megakaryocyte, CFU-granulocyte/ macrophage, CFU-macrophage, and CFU-granulocyte). Femurs and vertebrae were subjected to micro-CT analyses, biomechanical testing, and F, Mg, and Ca content assays. DF was evaluated using quantitative fluorescence and clinical criteria. Strain-specific responses to F were observed for DF, serum studies, ex vivo cell culture studies, and bone quality. Among the strains, there were no patterns or significant correlations between DF severity and the actions of F on bone homeostasis (serum studies, ex vivo assays, or bone quality parameters). The genetic background continues to play a role in the actions of F on tooth enamel development and bone homeostasis. F exposure led to variable phenotypic responses between strains involving dental enamel development and bone metabolism.

Original languageEnglish (US)
Pages (from-to)261-267
Number of pages7
JournalCells Tissues Organs
Volume194
Issue number2-4
DOIs
StatePublished - Aug 2011

Fingerprint

Dental Fluorosis
Inbred Strains Mice
Fluorides
Bone and Bones
Dental Enamel
Granulocytes
Homeostasis
Bone Development
Macrophages
Cell Culture Techniques
Serum
Physiologic Calcification
Megakaryocytes
Osteoclasts
Osteoblasts
Osteogenesis
Drinking Water
Bone Marrow Cells
Femur
Tooth

Keywords

  • Bone
  • Fluoride
  • Fluorosis
  • Genetics
  • Teeth

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Yan, D., Willett, T. L., Gu, X. M., Martinez Mier, E., Sardone, L., McShane, L., ... Everett, E. T. (2011). Phenotypic variation of fluoride responses between inbred strains of mice. Cells Tissues Organs, 194(2-4), 261-267. https://doi.org/10.1159/000324224

Phenotypic variation of fluoride responses between inbred strains of mice. / Yan, Dong; Willett, Thomas L.; Gu, Xiao Mei; Martinez Mier, Esperanza; Sardone, Laura; McShane, Lauren; Grynpas, Marc; Everett, Eric T.

In: Cells Tissues Organs, Vol. 194, No. 2-4, 08.2011, p. 261-267.

Research output: Contribution to journalArticle

Yan, D, Willett, TL, Gu, XM, Martinez Mier, E, Sardone, L, McShane, L, Grynpas, M & Everett, ET 2011, 'Phenotypic variation of fluoride responses between inbred strains of mice', Cells Tissues Organs, vol. 194, no. 2-4, pp. 261-267. https://doi.org/10.1159/000324224
Yan, Dong ; Willett, Thomas L. ; Gu, Xiao Mei ; Martinez Mier, Esperanza ; Sardone, Laura ; McShane, Lauren ; Grynpas, Marc ; Everett, Eric T. / Phenotypic variation of fluoride responses between inbred strains of mice. In: Cells Tissues Organs. 2011 ; Vol. 194, No. 2-4. pp. 261-267.
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