Phosphatase activity, trimerization, and the C-terminal polybasic region are all required for PRL1-mediated cell growth and migration

Jin Peng Sun, Yong Luo, Xiao Yu, Wei Qing Wang, Bo Zhou, Fubo Liang, Zhong-Yin Zhang

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The phosphatase of regenerating liver (PRL) phosphatases are implicated in a number of tumorigenesis and metastasis processes. The PRLs are unique among protein-tyrosine phosphatases in that they have extremely low phosphatase activity, a high propensity for trimer formation, and a polybasic region that precedes the C-terminal prenylation motif. To investigate the functional significance of these distinctive biochemical and structural features, we established a cell-based system in which ectopic PRL1 expression increased cell proliferation and migration, whereas knockdown of endogenous PRL1 abrogated these cellular activities. We showed that the intrinsic PRL1 phosphatase activity is obligatory for its biological function. We provided evidence that trimerization may be a general property for all PRL enzymes, and that PRL1 trimer formation is essential for the PRL1-mediated cell growth and migration. This finding indicates a novel mechanism for phosphatase regulation. We further demonstrated that the conserved C-terminal polybasic region is important for specific phosphoinositide recognition by PRL1. Both the polybasic residues and the adjacent prenylation motif are required for proper PRL1 subcellular localization and full biological activity.

Original languageEnglish
Pages (from-to)29043-29051
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number39
DOIs
StatePublished - Sep 28 2007

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Cell growth
Phosphoric Monoester Hydrolases
Cell Movement
Growth
Prenylation
Liver
Protein Tyrosine Phosphatases
Cell proliferation
Phosphatidylinositols
Bioactivity
Carcinogenesis
Cell Proliferation
Neoplasm Metastasis
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Phosphatase activity, trimerization, and the C-terminal polybasic region are all required for PRL1-mediated cell growth and migration. / Sun, Jin Peng; Luo, Yong; Yu, Xiao; Wang, Wei Qing; Zhou, Bo; Liang, Fubo; Zhang, Zhong-Yin.

In: Journal of Biological Chemistry, Vol. 282, No. 39, 28.09.2007, p. 29043-29051.

Research output: Contribution to journalArticle

Sun, Jin Peng ; Luo, Yong ; Yu, Xiao ; Wang, Wei Qing ; Zhou, Bo ; Liang, Fubo ; Zhang, Zhong-Yin. / Phosphatase activity, trimerization, and the C-terminal polybasic region are all required for PRL1-mediated cell growth and migration. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 39. pp. 29043-29051.
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AU - Zhou, Bo

AU - Liang, Fubo

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