Phosphatase of regenerating liver 3 (prl3) provokes a tyrosine phosphoproteome to drive prometastatic signal transduction

Chad D. Walls, Anton Iliuk, Yunpeng Bai, Mu Wang, Andy Tao, Zhong Yin Zhang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Phosphatase of regenerating liver 3 (PRL3) is suspected to be a causative factor toward cellular metastasis when in excess. To date, the molecular basis for PRL3 function remains an enigma, making efforts at distilling a concerted mechanism for PRL3-mediated metastatic dissemination very difficult. We previously discovered that PRL3 expressing cells exhibit a pronounced increase in protein tyrosine phosphorylation. Here we take an unbiased mass spectrometry-based approach toward identifying the phosphoproteins exhibiting enhanced levels of tyrosine phosphorylation with a goal to define the "PRL3-mediated signaling network." Phosphoproteomic data support intracellular activation of an extensive signaling network normally governed by extracellular ligand-activated transmembrane growth factor, cytokine, and integrin receptors in the PRL3 cells. Additionally, data implicate the Src tyrosine kinase as the major intracellular kinase responsible for "hijacking" this network and provide strong evidence that aberrant Src activation is a major consequence of PRL3 overexpression. Importantly, the data support a PDGF(α/β)-, Eph (A2/B3/B4)-, and Integrin (α1/ α5)-receptor array as being the predominant network coordinator in the PRL3 cells, corroborating a PRL3-induced mesenchymal-state. Within this network, we find that tyrosine phosphorylation is increased on a multitude of signaling effectors responsible for Rho-family GTPase, PI3K-Akt, STAT, and ERK activation, linking observations made by the field as a whole under Src as a primary signal transducer. Our phosphoproteomic data paint the most comprehensive picture to date of how PRL3 drives prometastatic molecular events through Src activation.

Original languageEnglish (US)
Pages (from-to)3759-3777
Number of pages19
JournalMolecular and Cellular Proteomics
Volume12
Issue number12
DOIs
StatePublished - Dec 1 2013

Fingerprint

Signal transduction
Phosphoric Monoester Hydrolases
Liver
Tyrosine
Signal Transduction
Phosphorylation
Chemical activation
Integrins
rho GTP-Binding Proteins
Cytokine Receptors
Paint
src-Family Kinases
GTP Phosphohydrolases
Phosphoproteins
Transducers
Phosphatidylinositol 3-Kinases
varespladib methyl
Mass spectrometry
Mass Spectrometry
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Cite this

Phosphatase of regenerating liver 3 (prl3) provokes a tyrosine phosphoproteome to drive prometastatic signal transduction. / Walls, Chad D.; Iliuk, Anton; Bai, Yunpeng; Wang, Mu; Tao, Andy; Zhang, Zhong Yin.

In: Molecular and Cellular Proteomics, Vol. 12, No. 12, 01.12.2013, p. 3759-3777.

Research output: Contribution to journalArticle

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