Phosphate transport after acute changes in total NAD content in renal proximal tubules

P. I. Campbell, M. I. Abraham, Jesus Dominguez, J. A. McAteer, S. A. Kempson

Research output: Contribution to journalArticle

Abstract

Suspensions of proximal tubules were obtained by collagenase digestion of rat renal cortex followed by centrifugation on a percoll gradient. NAD content in tubules incubated at 37°C was decreased by 40-60% compared with tubules incubated at 4°C. This change occurred within 30 min and was maintained for up to 2 hr. Inhibitors of NAD hydrolysing enzymes prevented the depletion of cellular NAD at 37°C. Acute changes in proximal tubule NAD content at 37°C were not accompanied by changes in phosphate uptake by brush border membrane vesicles subsequently prepared from the same tubules. In contrast, incubation of tubules with parathyroid hormone (10-6 M) produced the expected inhibition (20%) of brush border membrane transport of phosphate. One implication of these findings is that acute changes in total NAD content of proximal tubules at 37°C may not influence the phosphate transport system in the renal brush border membrane. Other interpretations are discussed.

Original languageEnglish
Pages (from-to)253-258
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume193
Issue number4
StatePublished - 1990

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Proximal Kidney Tubule
NAD
Phosphates
Brushes
Microvilli
Membranes
Kidney
Centrifugation
Collagenases
Parathyroid Hormone
Rats
Digestion
Suspensions
Enzymes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Phosphate transport after acute changes in total NAD content in renal proximal tubules. / Campbell, P. I.; Abraham, M. I.; Dominguez, Jesus; McAteer, J. A.; Kempson, S. A.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 193, No. 4, 1990, p. 253-258.

Research output: Contribution to journalArticle

Campbell, P. I. ; Abraham, M. I. ; Dominguez, Jesus ; McAteer, J. A. ; Kempson, S. A. / Phosphate transport after acute changes in total NAD content in renal proximal tubules. In: Proceedings of the Society for Experimental Biology and Medicine. 1990 ; Vol. 193, No. 4. pp. 253-258.
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