Phosphatidic acid induces calcium influx in neutrophils via verapamil- sensitive calcium channels

Rafat A. Siddiqui, Daniel J. Burtschi, Richard Kovacs

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Phosphatidic acid (PA) induces a biphasic Ca2+ mobilization response in human neutrophils. The initial increase is due to the mobilization of Ca2+ from intracellular stores, whereas the secondary increase is due to the influx of Ca2+ from extracellular sources. The present investigation characterizes PA-induced Ca2+ influx in neutrophils. Depolarization of neutrophils by 50 mM KCl enhanced PA-induced Ca2+ influx, whereas verapamil, a Ca2+ channel blocker, attenuated this response in a dose- dependent manner. These observations suggest that PA-induced Ca2+ influx is mediated via verapamil-sensitive Ca2+ channels. Stimulation of neutrophils with exogenous PA results in accumulation of endogenously generated PA with a time course similar to the effects of exogenous PA on Ca2+ influx. Ethanol inhibited the accumulation of endogenous PA and calcium mobilization, indicating that activation of membrane phospholipase D plays a role in PA- mediated Ca2+ influx. The results of this study suggest that exogenously added PA stimulates the generation of intracellular PA, which then mediates Ca2+ influx through verapamil-sensitive Ca2+ channels. (C) 2000 Wiley- Liss, Inc.

Original languageEnglish (US)
Pages (from-to)297-304
Number of pages8
JournalJournal of Cellular Biochemistry
Volume78
Issue number2
DOIs
StatePublished - Jul 10 2000

Keywords

  • Calcium influx
  • Neutrophils
  • Phosphatidic acid
  • Verapamil

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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