Phosphatidylinositol 3-kinase and dynamics of insulin resistance in denervated slow and fast muscles in vivo

Jeffrey S. Elmendorf, Alice Damrau-Abney, Terry R. Smith, Tonya S. David, Jiri Turinsky

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Regulation of glucose uptake by 1- and 3-day denervated soleus (slow- twitch) and plantaris (fast-twitch) muscles in vivo was investigated. One day after denervation, soleus and plantaris muscles exhibited 62 and 65% decreases in insulin-stimulated 2-deoxyglucose uptake, respectively, compared with corresponding control muscles. At this interval, denervated muscles showed no alterations in insulin receptor binding and activity, amount and activity of phosphatidylinositol 3-kinase, and amounts of GLUT-1 and GLUT-4. Three days after denervation, there was no increase in 2-deoxyglucose uptake in response to insulin in soleus muscle, whereas plantaris muscle exhibited a 158% increase in basal and an almost normal absolute increment in insulin- stimulated uptake. Despite these differences, denervated soleus and plantaris muscles exhibited comparable decreases in insulin-stimulated activities of the insulin receptor (~40%) and phosphatidylinositol 3-kinase (~50%) and a pronounced decrease in GLUT-4. An increase in GLUT-1 in plantaris, but not soleus, muscle 3 days after denervation is consistent with augmented basal 2- deoxyglucose uptake in plantaris muscle at this interval. These results demonstrate that, in denervated muscles, there is a clear dissociation between insulin-stimulated 2-deoxyglucose uptake and upstream events involved in insulin-stimulated glucose uptake.

Original languageEnglish (US)
Pages (from-to)E661-E670
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume272
Issue number4 35-4
StatePublished - Apr 1 1997

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Keywords

  • 2-deoxy-D-glucose uptake by muscle
  • glucose transporters
  • insulin receptor
  • rats
  • single hindlimb denervation

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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