Phospholemman expression induces a hyperpolarization-activated chloride current in Xenopus oocytes

J. R. Moorman, C. J. Palmer, J. E. John, M. E. Durieux, L. R. Jones

Research output: Contribution to journalArticle

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Abstract

A new type of chloride channel has been identified by functional expression of phospholemman, a 72-amino acid cardiac sarcolemmal protein with a single transmembrane domain. Xenopus oocytes injected with phospholemman RNA developed a chloride-selective current, which was activated by hyperpolarizing pulses. The current activated very slowly with a pronounced sigmoidal delay, did not inactivate, and increased in amplitude with trains of pulses, depolarized holding potentials, and low extracellular pH. Point mutations within the single transmembrane region abolished the sigmoidal delay of expressed currents. Phospholemman appears to be the smallest plasma membrane channel protein yet known. The structure is dissimilar to any chloride channel described thus far.

Original languageEnglish (US)
Pages (from-to)14551-14554
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number21
StatePublished - Aug 11 1992

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Xenopus
Oocytes
Chlorides
Chloride Channels
Cell membranes
Ion Channels
Point Mutation
Blood Proteins
Membrane Proteins
Proteins
Cell Membrane
RNA
Amino Acids
phospholemman

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Phospholemman expression induces a hyperpolarization-activated chloride current in Xenopus oocytes. / Moorman, J. R.; Palmer, C. J.; John, J. E.; Durieux, M. E.; Jones, L. R.

In: Journal of Biological Chemistry, Vol. 267, No. 21, 11.08.1992, p. 14551-14554.

Research output: Contribution to journalArticle

Moorman, J. R. ; Palmer, C. J. ; John, J. E. ; Durieux, M. E. ; Jones, L. R. / Phospholemman expression induces a hyperpolarization-activated chloride current in Xenopus oocytes. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 21. pp. 14551-14554.
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