Phosphorylation by casein kinase II affects the interaction of caldesmon with smooth muscle myosin and tropomyosin

Natalia Bogatcheva, A. V. Vorotnikov, K. G. Birukov, V. P. Shirinsky, N. B. Gusev

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Smooth muscle caldesmon was phosphorylated by casein kinase II, and the effects of phosphorylation on the interaction of caldesmon and its chymotryptic peptides with myosin and tropomyosin were investigated. The N-terminal chymotryptic peptide of caldesmon of molecular mass 27 kDa interacted with myosin. Phosphorylation of Ser-73 catalysed by casein kinase II resulted in a 2-fold decrease in the affinity of the native caldesmon (or its 27 kDa N-terminal peptide) for smooth muscle myosin. At low ionic strength, caldesmon and its N-terminal peptides of molecular masses 25 and 27 kDa were retarded on a column of immobilized tropomyosin. Phosphorylation of Ser-73 led to a 2-4-fold decrease in the affinity of caldesmon (or its N-terminal peptides) for tropomyosin. Thus phosphorylation of Ser-73 catalysed by casein kinase II affects the interaction of caldesmon with both smooth muscle myosin and tropomyosin.

Original languageEnglish (US)
Pages (from-to)437-442
Number of pages6
JournalBiochemical Journal
Volume290
Issue number2
StatePublished - 1993
Externally publishedYes

Fingerprint

Smooth Muscle Myosins
Calmodulin-Binding Proteins
Casein Kinase II
Tropomyosin
Phosphorylation
Peptides
Molecular mass
Myosins
Ionic strength
Osmolar Concentration
Smooth Muscle
Muscle

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bogatcheva, N., Vorotnikov, A. V., Birukov, K. G., Shirinsky, V. P., & Gusev, N. B. (1993). Phosphorylation by casein kinase II affects the interaction of caldesmon with smooth muscle myosin and tropomyosin. Biochemical Journal, 290(2), 437-442.

Phosphorylation by casein kinase II affects the interaction of caldesmon with smooth muscle myosin and tropomyosin. / Bogatcheva, Natalia; Vorotnikov, A. V.; Birukov, K. G.; Shirinsky, V. P.; Gusev, N. B.

In: Biochemical Journal, Vol. 290, No. 2, 1993, p. 437-442.

Research output: Contribution to journalArticle

Bogatcheva, N, Vorotnikov, AV, Birukov, KG, Shirinsky, VP & Gusev, NB 1993, 'Phosphorylation by casein kinase II affects the interaction of caldesmon with smooth muscle myosin and tropomyosin', Biochemical Journal, vol. 290, no. 2, pp. 437-442.
Bogatcheva, Natalia ; Vorotnikov, A. V. ; Birukov, K. G. ; Shirinsky, V. P. ; Gusev, N. B. / Phosphorylation by casein kinase II affects the interaction of caldesmon with smooth muscle myosin and tropomyosin. In: Biochemical Journal. 1993 ; Vol. 290, No. 2. pp. 437-442.
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