Phosphorylation of Irs1 at SER-522 inhibits insulin signaling

Jodel Giraud, Michael Haas, Edward P. Feener, Kyle D. Copps, X. Dong, Sarah L. Dunn, Morris F. White

Research output: Contribution to journalArticle

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Abstract

Multisite phosphorylation of Irs1 on serine and threonine residues regulates insulin signaling that can contribute to insulin resistance. We identified by mass spectrometry the phosphorylation of Ser522 in rat Irs1 (S522Irs1). The functional effects of this phosphorylation site were investigated in cultured cells using a sequence-specific phosphoserine antibody. Insulin stimulated the phosphorylation of S522Irs1 in L6 myoblasts and myotubes. S522Irs1 phosphorylation was inhibited by wortmannin, whereas PD98059, rapamycin, or glucose-starvation had no effect. Reducing Akt expression with small interfering RNA inhibited insulin-stimulated phosphorylation of S522Irs1, suggesting the involvement of the phosphatidylinositol 3-kinase→ Akt cascade. A S522Irs1→ A522Irs1 substitution increased insulin-stimulated tyrosine phosphorylation of Irs1 and signaling, whereas a S522Irs1→ E522Irs1 substitution reduced insulin-stimulated Irs1 tyrosine phosphorylation. Together, these results suggest the phosphatidylinositol 3-kinase→Akt cascade can inhibit insulin signaling through the phosphorylation of S522Irs1.

Original languageEnglish (US)
Pages (from-to)2294-2302
Number of pages9
JournalMolecular Endocrinology
Volume21
Issue number9
DOIs
StatePublished - Sep 2007
Externally publishedYes

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Phosphorylation
Insulin
Tyrosine
Phosphatidylinositol 3-Kinase
Phosphoserine
Myoblasts
Skeletal Muscle Fibers
Sirolimus
Threonine
Starvation
Phosphatidylinositols
Serine
Small Interfering RNA
Insulin Resistance
Cultured Cells
Mass Spectrometry
Glucose
Antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Giraud, J., Haas, M., Feener, E. P., Copps, K. D., Dong, X., Dunn, S. L., & White, M. F. (2007). Phosphorylation of Irs1 at SER-522 inhibits insulin signaling. Molecular Endocrinology, 21(9), 2294-2302. https://doi.org/10.1210/me.2007-0159

Phosphorylation of Irs1 at SER-522 inhibits insulin signaling. / Giraud, Jodel; Haas, Michael; Feener, Edward P.; Copps, Kyle D.; Dong, X.; Dunn, Sarah L.; White, Morris F.

In: Molecular Endocrinology, Vol. 21, No. 9, 09.2007, p. 2294-2302.

Research output: Contribution to journalArticle

Giraud, J, Haas, M, Feener, EP, Copps, KD, Dong, X, Dunn, SL & White, MF 2007, 'Phosphorylation of Irs1 at SER-522 inhibits insulin signaling', Molecular Endocrinology, vol. 21, no. 9, pp. 2294-2302. https://doi.org/10.1210/me.2007-0159
Giraud, Jodel ; Haas, Michael ; Feener, Edward P. ; Copps, Kyle D. ; Dong, X. ; Dunn, Sarah L. ; White, Morris F. / Phosphorylation of Irs1 at SER-522 inhibits insulin signaling. In: Molecular Endocrinology. 2007 ; Vol. 21, No. 9. pp. 2294-2302.
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