Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A

Frank Eckerdt, Gaetan Pascreau, Meridee Phistry, Andrea L. Lewellyn, Anna De Paoli-Roach, James L. Maller

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Entry into mitosis requires the activation of mitotic kinases, including Aurora A and Polo-like kinase 1 (Plk1). Increased levels of these kinases are frequently found associated with human cancers, and therefore it is imperative to understand the processes leading to their activation. We demonstrate that TPX2, but neither Ajuba nor Inhibitor-2, can activate Aurora A directly. Moreover, Plx1 can induce Aurora A T-loop phosphorylation indirectly in vivo during oocyte maturation. We identify Ser204 in TPX2 as a Plx1 phosphorylation site. Mutating Ser204 to alanine decreases activation of Aurora A, whereas a phosphomimetic Asp mutant exhibits enhanced activating ability. Finally, we show that phosphorylation of TPX2 with Plx1 increases its ability to activate Aurora A. Taken together, our data indicate that Plx1 promotes activation of Aurora A, most likely through TPX2. In light of the current literature, we propose a model in which Plx1 and Aurora A activate each other in a positive feedback loop.

Original languageEnglish
Pages (from-to)2413-2419
Number of pages7
JournalCell Cycle
Volume8
Issue number15
StatePublished - Aug 1 2009

Fingerprint

Phosphorylation
Aurora Kinase A
Mitosis
Alanine
Oocytes
Phosphotransferases
Neoplasms
polo-like kinase 1

Keywords

  • Ajuba
  • Aurora kinase
  • Mitosis
  • Oocyte maturation
  • Phosphatase inhibitor-2
  • Polo-like kinase
  • TPX2
  • Xenopus laevis

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Eckerdt, F., Pascreau, G., Phistry, M., Lewellyn, A. L., De Paoli-Roach, A., & Maller, J. L. (2009). Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A. Cell Cycle, 8(15), 2413-2419.

Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A. / Eckerdt, Frank; Pascreau, Gaetan; Phistry, Meridee; Lewellyn, Andrea L.; De Paoli-Roach, Anna; Maller, James L.

In: Cell Cycle, Vol. 8, No. 15, 01.08.2009, p. 2413-2419.

Research output: Contribution to journalArticle

Eckerdt, F, Pascreau, G, Phistry, M, Lewellyn, AL, De Paoli-Roach, A & Maller, JL 2009, 'Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A', Cell Cycle, vol. 8, no. 15, pp. 2413-2419.
Eckerdt F, Pascreau G, Phistry M, Lewellyn AL, De Paoli-Roach A, Maller JL. Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A. Cell Cycle. 2009 Aug 1;8(15):2413-2419.
Eckerdt, Frank ; Pascreau, Gaetan ; Phistry, Meridee ; Lewellyn, Andrea L. ; De Paoli-Roach, Anna ; Maller, James L. / Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A. In: Cell Cycle. 2009 ; Vol. 8, No. 15. pp. 2413-2419.
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