Phosphorylation regulates RNA binding by the human T-cell leukemia virus rex protein

Patrick L. Green, Michele Yip-Schneider, Yiming Xie, Irvin S Y Chen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The Rex protein of human T-cell leukemia virus types I (HTLV-I) and II (HTLV-II) regulates the expression of the viral structural genes and is critical for viral replication. Rex acts by specifically binding to RNAs containing sequences of the R region of the 5′ long terminal repeat. Two forms of Rex detected in HTLV-II-infected cells, p26rex and p24rex, differ in the extent of serine phosphorylation. Two-dimensional phosphopeptide analysis indicates that p26rex is extensively phosphorylated at multiple sites. Using a sensitive immunobinding assay, we show that the phosphorylation state of Rex determines the efficiency of binding of Rex to HTLV-II target RNAs. Thus, the phosphorylation state of Rex in the infected cell may be a switch that determines whether virus exists in a latent or productive state. These studies also suggest that phosphorylation of RNA-binding regulatory proteins is a more general mechanism of gene regulation.

Original languageEnglish (US)
Pages (from-to)4325-4330
Number of pages6
JournalJournal of Virology
Volume66
Issue number7
StatePublished - Jul 1992
Externally publishedYes

Fingerprint

rex Gene Products
Deltaretrovirus
Human T-lymphotropic virus 2
phosphorylation
Phosphorylation
RNA
Human T-lymphotropic virus 1
Primate T-lymphotropic virus 1
proteins
Phosphopeptides
RNA-binding proteins
terminal repeat sequences
RNA-Binding Proteins
Viral Genes
Terminal Repeat Sequences
structural genes
regulatory proteins
virus replication
serine
Serine

ASJC Scopus subject areas

  • Immunology

Cite this

Phosphorylation regulates RNA binding by the human T-cell leukemia virus rex protein. / Green, Patrick L.; Yip-Schneider, Michele; Xie, Yiming; Chen, Irvin S Y.

In: Journal of Virology, Vol. 66, No. 7, 07.1992, p. 4325-4330.

Research output: Contribution to journalArticle

Green, Patrick L. ; Yip-Schneider, Michele ; Xie, Yiming ; Chen, Irvin S Y. / Phosphorylation regulates RNA binding by the human T-cell leukemia virus rex protein. In: Journal of Virology. 1992 ; Vol. 66, No. 7. pp. 4325-4330.
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