Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons

Nader D. Halim, Thomas Mcfate, Ahmed Mohyeldin, Peter Okagaki, Lioubov G. Korotchkina, Mulchand S. Patel, Nam Ho Jeoung, Robert Harris, Michael J. Schell, Ajay Verma

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Glucose metabolism in nervous tissue has been proposed to occur in a compartmentalized manner with astrocytes contributing largely to glycolysis and neurons being the primary site of glucose oxidation. However, mammalian astrocytes and neurons both contain mitochondria, and it remains unclear why in culture neurons oxidize glucose, lactate, and pyruvate to a much larger extent than astrocytes. The objective of this study was to determine whether pyruvate metabolism is differentially regulated in cultured neurons versus astrocytes. Expression of all components of the pyruvate dehydrogenase complex (PDC), the rate-limiting step for pyruvate entry into the Krebs cycle, was determined in cultured astrocytes and neurons. In addition, regulation of PDC enzymatic activity in the two cell types via protein phosphorylation was examined. We show that all components of the PDC are expressed in both cell types in culture, but that PDC activity is kept strongly inhibited in astrocytes through phosphorylation of the pyruvate dehydrogenase alpha subunit (PDHα). In contrast, neuronal PDC operates close to maximal levels with much lower levels of phosphorlyated PDHα. Dephosphorylation of astrocytic PDHα restores PDC activity and lowers lactate production. Our findings suggest that the glucose metabolism of astrocytes and neurons may be far more flexible than previously believed.

Original languageEnglish
Pages (from-to)1168-1176
Number of pages9
JournalGLIA
Volume58
Issue number10
DOIs
StatePublished - Aug 1 2010

Fingerprint

Pyruvate Dehydrogenase Complex
Pyruvic Acid
Astrocytes
Oxidoreductases
Phosphorylation
Phenotype
Neurons
Brain
Glucose
Lactic Acid
Nerve Tissue
Citric Acid Cycle
Glycolysis
Mitochondria
Cell Culture Techniques

Keywords

  • Dichloroacetate
  • Glycolysis
  • Lactate
  • Oxidation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Halim, N. D., Mcfate, T., Mohyeldin, A., Okagaki, P., Korotchkina, L. G., Patel, M. S., ... Verma, A. (2010). Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons. GLIA, 58(10), 1168-1176. https://doi.org/10.1002/glia.20996

Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons. / Halim, Nader D.; Mcfate, Thomas; Mohyeldin, Ahmed; Okagaki, Peter; Korotchkina, Lioubov G.; Patel, Mulchand S.; Jeoung, Nam Ho; Harris, Robert; Schell, Michael J.; Verma, Ajay.

In: GLIA, Vol. 58, No. 10, 01.08.2010, p. 1168-1176.

Research output: Contribution to journalArticle

Halim, ND, Mcfate, T, Mohyeldin, A, Okagaki, P, Korotchkina, LG, Patel, MS, Jeoung, NH, Harris, R, Schell, MJ & Verma, A 2010, 'Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons', GLIA, vol. 58, no. 10, pp. 1168-1176. https://doi.org/10.1002/glia.20996
Halim, Nader D. ; Mcfate, Thomas ; Mohyeldin, Ahmed ; Okagaki, Peter ; Korotchkina, Lioubov G. ; Patel, Mulchand S. ; Jeoung, Nam Ho ; Harris, Robert ; Schell, Michael J. ; Verma, Ajay. / Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons. In: GLIA. 2010 ; Vol. 58, No. 10. pp. 1168-1176.
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